花生青枯菌红安分离物的鉴定

Identification and mutation of Ralstonia solacearum Hongan isolate

从湖北红安发病花生根部分离到一个细菌分离物2C1,对该分离物进行了TZC显色反应,致病力的测定,16S rRNA、23S rRNA和鞭毛蛋白基因filC的PCR扩增,16S rRNA PCR扩增产物的序列分析。结果显示,分离物2C1在TZC培养基上表现出青枯菌的典型菌落形态,对花生具有强致病性,PCR扩增获得与预期大小的产物片段;16S rRNA核苷酸序列具有与其它青枯菌分离物99%左右的一致性。上述结果表明:2C1是具有强致病力的花生青枯菌分离物。

A bacterium isolate 2C1 from the root of diseased peanut seedling from Hubei Hongan was identified by its pathogenecity to peanut,PCR amplification of 16S rRNA,23S rRNA and filC gene and 16S rRNA PCR product sequencing.The results revealed that 2C1 was virulent to peanut and showed the typical bacterial wilt symptom,and it amplified the prospected size of fragments of 16S rRNA,23S rRNA and filC gene of Ralstonia solanacearum by PCR,and its 16S rRNA PCR product showed 99% nucleotide matching with those strains of R.solanacearum.