摘要
基于碱性介质中三聚氰胺对鲁米诺-铁氰化钾化学发光体系的抑制作用,首次建立了三聚氰胺的毛细管电泳-化学发光分析法。在优化的实验条件下,该方法的线性范围和检出限分别为0.5～100μg/L和0.12μg/L,对0.5μg/L三聚氰胺的相对标准偏差为1.97﹪(n=5)。该方法应用于加标尿样中三聚氰胺含量的测定,结果令人满意。
A quick and sensitive method for the detection of melamine in alkaline medium has been developed by capillary electrophoresis-chemiluminescence based on decreasing effect of melamine for luminal-potassium ferricyanide system.Under the optimum conditions,the CL signal was proportional to the concentration of melamine in the range of 0.5-100 μg/L,and the detection limit（S/N = 3） was 0.12 μg/L.For the determination of 0.5 μg/L melamine solution,the RSD is 1.97 ﹪（n = 5）.The proposed method was applied to the determination of melamine in spiked urine samples and the results were satisfactory.
出处
《湖北师范学院学报（自然科学版）》
2010年第1期67-71,共5页
Journal of Hubei Normal University(Natural Science)
基金
教育部重点项目(206096)
湖北省教育厅创新团队项目和重点项目(D200922005)资助
关键词
三聚氰胺
检测
毛细管电泳
化学发光
melamine
detection
capillary electrophoresis
chemiluminescence
