脂蛋白相关磷脂酶A_2活性与兔动脉粥样硬化的关系以及辛伐他汀干预作用的研究

Lipoprotein-associated phospholipase A2 activity as related to atherosclerosis in rabbits and the effects of simvastatin intervention

目的利用高脂喂饲家兔诱发兔动脉粥样硬化模型,探讨脂蛋白相关磷脂酶A2(Lp-PLA2)活性与动脉粥样硬化发病的关系,Lp-PLA2和传统炎症因子高敏C反应蛋白(hs-CRP)在预测动脉粥样硬化发病方面的异同,以及辛伐他汀对动脉粥样硬化的影响。方法56只健康雄性新西兰白兔随机分为3组,健康对照组(24只),高脂模型组(24只),辛伐他汀组(8只),造模的同时给药8周,3组分别于4、8、12周测Lp-PLA2和hs-CRP的含量。结果高脂模型组Lp-PLA2活性和hs-CRP浓度8、12周时与健康对照组相比均明显升高,辛伐他汀组Lp-PLA2活性和hs-CRP浓度12周时明显低于高脂模型组。结论动脉粥样硬化与Lp-PLA2活性密切相关,辛伐他汀有抗动脉粥样硬化的作用。

Objective In models of atherosclerosis established with rabbits on high-fat diet, we investigated the lipoprotein-associated phospholipase A2 （Lp--PLA2） activity as related to atherosclerosis, whether Lp-PLA2 was predictive of atherosclerosis as compared to the classical inflammatory factor （high sensitivity C-reactive protein）, and the effects of simvastatin intervention on atherosclerosis. Methods Fifty-six healthy male New-Zealand rabbits were divided into the normal control group （n=24）, high-fat model group （n=24） and three simvastatin-treated groups （n=8 each, on an 8-week therapy, determined for levels of Lp-PLA2 and high sensitivity C-reactive protein at 4, 8 and 12 weeks）. Results Levels of Lp-PLA2 activity and high sensitivity C-reactive protein were higher in the high-fat model group than in the normal controls at 8 and 12 weeks, and lower in simvastatin-treated groups than in high-fat model group at 12 weeks. Conclusion Atberosclerosis may be closely related to lipoprotein-associated phospholipase A2 activity. Simvastatin appeared protective against atherosclerosis.