氰酸钠对小鼠耐缺氧作用的实验研究

Study of effects of sodium cyanate on hypoxia tolerance in mice

目的观察氰酸钠(NaOCN)对小鼠急性缺氧存活时间的影响。方法雄性昆明种小鼠随机分为氰酸钠组和对照组。观察腹腔注射氰酸钠后小鼠密闭缺氧耐受时间、亚硝酸钠中毒存活时间、心肌缺氧后标准耐受时间及断头后张口动作持续时间,评价氰酸钠对小鼠缺氧耐受性的影响。结果腹腔注射200、250mg/kg氰酸钠2h后,小鼠密闭缺氧耐受时间较对照组显著延长(P<0.01);腹腔注射200mg/kg氰酸钠2h后,氰酸钠组小鼠亚硝酸钠中毒后的存活时间及注射异丙肾上腺素后的心肌缺氧后标准耐受时间都较对照组显著延长(P<0.01)。结论氰酸钠可显著提高小鼠对常压密闭缺氧、亚硝酸钠中毒和心肌缺氧的耐受能力。

Objective To observe the effects of sodium cyanate （NaOCN） on mouse survival time following acute hypoxia. Methods Male Kunming mice were randomly divided into two groups：NaOCN group and the control group. The hypoxic tolerance time under sealed condition, survival time following nitrite poisoning, standard tolerance time following myocardial hypoxia and gape duration following decapitation were recorded after the intraperitoneal injection of NaOCN in mice. Then the effects of NaOCN on mouse hypoxie tolerance were evaluated according to the results of above tests. Results At 2 h after the intraperitoneal injection of 200 or 250 mg/kg NaOCN,the hypoxic tolerance time of the mice under sealed condition significantly prolonged compared with the control group（P 〈 0.01 ）. At 2 h after the intraperitoneal injection of 200 mg/kg NaOCN ,the survival time of nitrite - poisoned mice and standard tolerance time of those injected isoprel thereafter also significantly prolonged compared with the control group （ P 〈 0.01 ）. Conclusion NaOCN can obviously increase the tolerance to hypoxia under sealed condition with normal pressure,nitrite poisoning and myocardial hypoxia.