创伤失血性休克家兔血浆中循环内皮细胞与一氧化氮及内皮素相关性研究

Study on Correlation between Circulating Endothelial Cells and Nitric Oxide and Endothelin in Hemorrhagic Shock Rabbits

目的探讨创伤失血性休克家兔血浆中一氧化氮(NO)、内皮素(ET)浓度及循环内皮细胞(CEC)数变化的相关性。方法 16只家兔,随机均分为创伤失血性休克组和对照组。创伤失血性休克组休克模型,经股动脉快速放血使动脉血压降至6.7kPa,维持90min,然后回输血液及等量的复方氯化钠。对照组处理同休克组,但未放血。两组分别在休克前、休克末、复苏末、复苏后45、90、180min同时测定血浆NO、ET和CEC水平变化。结果血浆NO水平在休克后逐渐升高,显著高于休克前和对照组水平(P<0.05或P<0.01),此后一直维持较高水平,180 min时达到高峰;休克后ET及CEC水平显著增加,并显著高于对照组及休克前(P<0.01),复苏后逐渐下降。结论创伤失血性休克后血浆NO、ET、CEC水平变化在其病理生理过程有一定意义。

Objective To study the correlation between circulating endothelial cell（CEC） and nitric oxide（NO） and endothelin （ET） in hemorrhagic shock rabbits. Methods Sixteen rabbits were randomly divided into hemorrhagic group and control group. The shock state （MAP = 6.7kPa） was induced by controlled bleeding and maintained for 90 min, then the shed blood was returned, followed by infusion of lactatic Ringer＇s solution, which is equal to the shed-blood volume. The animals in control group were treated as that in hemorrhage shock group without artificial bleeding. The contents of ET, NO and CEC in plasma were determined before, at the end of shock, immediately after resuscitation and at 45,90,180 min after resuscitation. Results There was a tendency of increase in NO in piglets after hemorrhagic shock. The contents of ET and CEC were markedly increased at the end of shock, and delined after resuscitation. Conclusion It is suggested that increased concen- tration of NO, ET and CEC in the plasma might play an important role in the pathogenesis of hemorrhagic shock.