抗寒锻炼期间影响白皮松针叶电阻抗图谱参数的生理指标

The physiological indices affecting parameters of electrical impedance spectroscopy in Pinus bungeana Zucc.needles during frost hardening

对北京十三陵苗圃北京蟒山、山西孝义和甘肃两当3个种源的8年生白皮松1年生针叶进行了电阻抗图谱(EIS)分析,测定各种源不经冷冻处理针叶的EIS参数,以及超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、可溶性蛋白质、脯氨酸、叶绿素和干物质含量等生理指标的变化;分析EIS参数和生理指标之间的相关性;用褐变法测定了针叶的抗寒性。结果表明:(1)针叶的EIS参数和生理指标随着抗寒性的提高发生变化,不同种源的EIS参数和某些生理指标在不同抗寒锻炼阶段,尤其在抗寒锻炼初期存在显著差异,并与不同种源抗寒性差异吻合;(2)EIS参数细胞膜时间恒量τm和胞外电阻re与针叶的叶绿素含量存在极显著负相关(r分别为-0.91和-0.68),β与针叶的干物质含量存在极显著负相关(r=-0.74),而胞内电阻ri和re与针叶的干物质含量存在极显著正相关(r分别为0.69和0.58),β与MDA含量存在极显著正相关(r=0.68);(3)β与褐变法求得的抗寒性存在极显著正相关(r=0.85),ri和re存在极显著负相关(r分别为-0.79和-0.66)。

The electrical impedance spectroscopy （EIS） was analyzed to one-year-old needles of Pinus bungeana Zucc.in an 8-year provenance field trial at the Nursery in Beijing Thirteen Tombs with provenances of Mangshan of Beijing, Xiaoyi of Shanxi, and Liangdang of Gansu. The parameters of EIS and the activities of superoxide dismutase （SOD）, the activities of peroxidase （POD）, the content of malondialdehyde （MDA）, the content of soluble protein, the content of proline, the chlorophyll content and the content of dry matter in non-frost exposed needles were tested with different provenances. The relations between EIS and physiological parameters were analyzed. The frost hardiness （FH） of needles was measured by means of the visual scoring of damage （VSD） after artificial freezing test. The results showed that： （1） Both the EIS parameters and the physiological parameters changed during frost hardening, and the EIS parameters and some physiological parameters differed significantly in certain stages of frost hardening, especially in the early stage, which was in accordance with the differences of FH in different provenances. （2） The EIS parameters membrane time constant τm and extracellular resistance re correlated negatively with the chlorophyll content （the correlation coefficient r were -0.91 and -0.68, respectively）, the β correlated negatively with the dry matter content （r=-0.74）, while the intracellular resistance ri and re had positive correlation with the dry matter content （r were 0.69 and 0.58, respectively）, as well as the β with the MDA content （r=0.68）. （3） The β correlated positively with the FH assessed by the VSD method （r=0.85）, whereas the ri and re did negatively （r were -0.79 and -0.66, respectively）.