NAA处理下南林-895杨无菌苗茎中糖含量及其相关酶活性的变化

The change of the sugar content and sugar-metabolizing enzymes activities in stems of Populus×euramericana ‘Nanlin895’aseptic seedling under NAA treatment

不同浓度NAA处理下,对南林-895杨无菌苗茎中糖含量及其相关酶活性的变化进行了测定和分析。结果表明:(1)100mg/L的NAA增加了南林-895杨无菌苗茎中蔗糖、果糖的含量,提高了酸性转化酶(AI)、中性转化酶(NI)的活性,降低了蔗糖磷酸合成酶(SPS)、蔗糖合成酶(SS)的活性,而高浓度NAA的作用效果正相反;(2)NAA处理下,茎中蔗糖、果糖含量均与AI活性显著正相关,蔗糖含量与SPS、SS活性极显著负相关,而果糖与之相关性不显著。可见,适宜浓度的NAA对南林-895杨无菌苗茎的糖代谢过程具有一定的促进作用。

The sugar content and sugar-metabolizing enzymes activities in stems of Populus×euramericana ‘Nanlin895’ aseptic seedling under different concentration stress of NAA were investigated, and also the correlations were analyzed. The results showed as follows： （1）The sucrose and fructose content were increased in stems of Populus×euramericana ‘Nanlin895’ aseptic seedling. Meanwhile, acid invertase （AI） and neutral invertase （NI） activities were enhanced. Besides, sucrose phosphate synthase （SPS） and sucrose synthase （SS） activities were reduced under the 100 mg/L NAA treatment. On the contrary, the role of higher concentration of NAA, which was higher than 100mg/L, was negative. （2）Under NAA treatment, the sucrose and fructose content were significant positive correlation with the activity of AI. Also there was significant negative correlation between sucrose content and the activities of SS and SPS, but the fructose content was not correlative with the SPS and SS activities. The fitting concentration of NAA would be taken definite promotion role in the process of sugar metabolism in the stems of Populus×euramericana ‘Nanlin895’ aseptic seedling.