STAT3在喉鳞状细胞癌及手术切缘组织中的表达及临床意义

The expression and clinical significance of STAT3 in laryngeal squamous cell carcinoma and surgical margins

目的:探讨信号转导子和转录激活子-3(signal transducer and activator of transcription3,STAT3)在喉癌组织中的表达与喉癌临床特征的关系,从细胞和基因水平探讨喉癌手术安全切缘的范围。方法:分别采用PV-9000免疫组织化学两步法及RT-PCR检测38例喉鳞癌组织及距癌组织边缘2、5、10mm组织中STAT3的表达及其mRNA转录情况,同时取距离癌组织边缘20mm以上的正常黏膜作为对照。结果:STAT3表达依癌组织→癌旁2mm→癌旁5mm→癌旁10mm→正常黏膜顺序递减。结论:STAT3在喉鳞癌组织中及癌旁各距离组织中的表达情况表明喉癌手术切除范围以距肿瘤边缘5mm作为安全切缘的标准较为适宜。

Objective：To study the expression of signal transducer and activator of transcription 3 （STAT3） in laryngeal carcinoma and explore the biological action of laryngeal tumor and the range of safe surgical margin. Methods：38 cases of laryngeal squamous cell carcinoma （LSCC） specimens and different regions adjacent carcinoma （2,5,10 mm） were examined for the expression level of STAT3 protein by IHC method （PV-9000）,and RT-PCR was employed to measure the expression of STAT3 mRNA. Normal mucosa （〉20 mm） was used as control. Results：The expression of STAT3 decreased as tissue pro-gressed in sequence from carcinoma tissues to 2,5,10 mm distant mucosa adjacent carcinoma and normal mucosa. Conclu-sion：It is appropriate to regard 5 mm away from tumors as resection margins for surgical treatment of laryngeal carcinoma.