摘要
目的:建立注射用脂溶性维生素(Ⅱ)中间品含量快速高效液相检测方法。方法:采用SemmetryRP-C18色谱柱(4.6mm×150mm,5μm),流动相:甲醇-水(梯度洗脱),流速:1.5ml/min,检测波长:265nm。结果:维生素A棕榈酸酯、维生素K1、维生素D2及维生素E浓度分别在0.144~0.216μg、0.240~0.360μg、8.000~12.000ng、14.560~21.840μg内呈良好的线性关系;平均回收率分别为99.74%(RSD=0.08%),98.49%(RSD=0.23%),99.83%(RSD=0.22%),99.30%(RSD=0.23%)。结论:该方法简便、快捷、灵敏,能有效控制产品质量。
Objective:To establish a method for the content determination of the mid-profection of fat soluble vitamins (Ⅱ) for Injection by RP-HPLC. Methods:Semmetry RP-C18 column (4.6 mm×150 mm,5 μm) was used. The mobile phases consisted of methanol and water (gradient elution),the flow rate was 1.5 ml/min and the detection wavelength was at 265 nm. Results:The method was proved to be linear when concentrations of vitamin A,K1,D2 and E were in the range of 0.144-0.216 μg,0.240-0.360 μg,8.000-12.000 ng,14.560-21.840 μg,respectively. The average recoveries and relative standard deviations of vitamin A,K1,D2 and E were 99.74% (RSD=0.08%),98.49% (RSD=0.23%),99.83% (RSD=0.22%)and 99.30% (RSD=0.23%),respectively. Conclusion:The method is simple,rapid,accurate and can be used for the quality control of the fat soluble vitamins for injection (Ⅱ).
出处
《中国医药导报》
CAS
2010年第10期82-83,共2页
China Medical Herald
关键词
反相色谱法
注射用脂溶性维生素
中间品
含量测定
RP-HPLC
The fat soluble vitamins (Ⅱ) for injection
Mid-profection
Content determination