舒胸片中人参皂苷Rg_1、人参皂苷Rb_1、三七皂苷R_1的含量测定

Content determination of ginsenoside Rg_1,ginsenoside Rb_1 and notoginsenoside R_1 in Shuxiong Tablets by RP-HPLC

目的:建立高效液相法同时测定舒胸片中人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1的含量测定方法。方法:采用Shim-pack-C18(4.6mm×250mm,5μm)色谱柱;流动相:乙腈-水进行梯度洗脱;检测波长:203nm;流速:1.0ml/min。结果:人参皂苷Rg1在0.82～8.20μg范围内与峰面积呈良好的线性关系(r=0.9998);人参皂苷Rb1在0.88～8.80μg范围内与峰面积呈良好的线性关系(r=0.9996);三七皂苷R1在0.32～3.20μg范围内与峰面积呈良好的线性关系(r=0.9998),总皂苷的平均回收率为99.76%,RSD=1.26%(n=6)。结论:本方法简便、准确、重现性好,可用于舒胸片中总皂苷的质量控制。

Objective：To establish a method for the content determination of ginsenoside Rg1,ginsenoside Rb1 and noto ginsenoside R1 in Shuxiong Tablets by RP-HPLC. Methods：The Shim-pack-C18 （4.6 mm×250 mm,5 μm） column was used. The mobile phases consisted of acetonitrile and water （gradient elution）,the flow rate was 1.0 ml/min and the detection wavelength was at 203 nm. Results：The linear ranges of ginsenoside Rg1 was at 0.82-8.20 μg; the linear ranges of ginsenoside Rb1 was at 0.88-8.80 μg; the linear ranges of notoginsenoside R1 was at 0.32-3.20 μg. The average recovery was 99.76% with a RSD of 1.26%. Conclusion：The method is fast,reliable,accurate and can be used in the quality con-trol of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside in Shuxiong Tablets.