庆大霉素C_(1a)高产菌株的推理育种的研究

Study on rational selection of high productivity strain of gentamicin C 1a

已有研究表明在氨基糖苷类抗生素的生物合成途径与细胞壁的合成途径间有一定的相关性。因而如能减小合成细胞壁的代谢流，则可促使氨基糖苷类抗生素高产。已知磷霉素是一种抑制细胞壁合成的抗生素。本文采用筛选对磷霉素敏感的菌株来获得庆大霉素Ｃ１ａ（ＧＭＣ１ａ）的高产株。结果表明在得到的６０株超敏菌株中，正变率为７３．３％。同时用薄层层析法和纸层析—生物显影法检测发酵液的组分情况，得到４株效价、组分均好的菌株（ＳＦ－０１－１、－１１、－１４、－６８）。稳定性试验表明这４株菌株稳定性良好，与亲株相比效价提高７５％～１０１％。用该法筛选可减小工作量，而且有效地提高了ＧＭＣ１ａ的产量已有研究表明在氨基糖苷类抗生素的生物合成途径与细胞壁的合成途径间有一定的相关性。因而如能减小合成细胞壁的代谢流，则可促使氨基糖苷类抗生素高产。已知磷霉素是一种抑制细胞壁合成的抗生素。本文采用筛选对磷霉素敏感的菌株来获得庆大霉素Ｃ１ａ（ＧＭＣ１ａ）的高产株。结果表明在得到的６０株超敏菌株中，正变率为７３．３％。同时用薄层层析法和纸层析—生物显影法检测发酵液的组分情况，得到４株效价、组分均好的菌株（ＳＦ－０１－１、－１１、－１４、－６８）。稳定性试验表明?

It had been found that there was the relation between the biosynthesis of the cell wall and the formation of aminoglycoside antibiotics. So the production of aminogycoside antibiotics increased when biosynthesis of the cell wall was inhibited. To obtain the higher producing strain of gentamicin C 1a , the method of screening the strain of super sensitive to fosfomycin was used in this experiment. Results showed that the ratio of forward mutant strains was 73.3% in 60 mutant strains. At the same time, the gentamicin C 1a component was detected by thin layer chromatography and paper chromatograthy bioautography. At last, four strains (SF 01 1, 11, 14 and 68) were selected because they had high productivity of gentamicin C 1a . Subculture test indicated that the heredity character of four strains was stable. Compared with parent strains, productivity of 4 strains increased by 75%￣101%.