球孢白僵菌MZ041016菌株对桔小实蝇的毒力测定

Detection on the Virulence of Beauveryia bassiana MZ041016 against Bactrocera dorsalis (Hendel)

以桔小实蝇成虫、幼虫和蛹为目标昆虫,利用不同分生孢子浓度的球孢白僵菌MZ041016菌株对其进行室内毒力测定。结果表明,供试菌株在室内对桔小实蝇成虫、幼虫和蛹具有较强的毒力。菌株分生孢子浓度为3.6×105个/mL时,可造成桔小实蝇各虫态大量死亡,在3.6×108个/mL孢子浓度下成虫死亡率达95.45%,致死中时间(LT50)为3.562d,第8天的致死中浓度(LC50)为2.14×105个/mL;在3.6×108个/mL孢子浓度下幼虫死亡率达95.94%,LT50为4.926d,第8天的LC50为3.03×105个/mL;3.6×108个/mL孢子浓度下蛹死亡率达78.15%,LT50为5.358d,第8天的LC50为3.31×105个/mL。

The virulence of five different concentrations of isolate of Beauveryia bassiana named MZ041016 against adult,larvae and pupae of Bactrocera dorsalis （Hendal） was tested in laboratory.Large amount of Bactrocera dorsalis （Hendal） of each stage were killed at the concentration 3.6×105 spores/mL.The mortality of 3.6×105 spores/mL against adult was 95.45%,and the lethal time（LT50） of this concentration was 3.562 d.The lethal concentration（LC50） of eight day after treatment was 2.14×105 spores/mL.The mortality of 3.6×108 spores/mL against larvae was 95.94%,and the lethal time（LT50） of this concentration was 4.926 d.The lethal concentration（LC50） of eight day after treatment was 3.03×105 spores/mL.The mortality of 3.6×108 spores/mL against pupae was 78.15%,and the lethal time（LT50） of this concentration was 5.358 d.The lethal concentration（LC50） of eight day after treatment was 3.31×105 spores/mL.The isolate of Beauveryia bassiana named MZ041016 had virulence against adult,larvae and pupae Bactrocera dorsalis （Hendal） in laboratory,and the application prospect of it in field should be feasible.The result provided a scientific basis for the pollution-free production of fruit and biology control of Bactrocera dorsalis （Hendal） in field.