外源蛋白酶水解白鲢内脏的工艺条件

Optimization of Enzymolysis Technology of Silver Carp Visceral Protein

为充分利用白鲢废弃物,利用碱性蛋白酶水解白鲢内脏,考察了温度、pH值、加酶量、料液比对白鲢内脏水解效果的影响。通过正交试验优化水解条件,结果表明:白鲢内脏水解的最佳条件是,酶解温度50℃,pH9.0,加酶量2000U/g,料液比1:3。在最佳酶解条件下水解7.5h,内脏蛋白质的水解度为41.73%,氮收率为64.76%。

Silver carp visceral protein was hydrolyzed by Alcalase.The optimum hydrolysis conditions for Alcalase are as follows：50 ℃,pH 9.0,ratio of Alcalase to fish protein being 2 000 U/g,ratio of material to water being 1∶3,7.5 hrs.The degree of hydrolysis and the recovery rate of protein was 41.73% and 64.76 %,respectively.