岷山红三叶异黄酮对产蛋后期母鸡生产性能、骨矿化及血液指标的影响

Effects of Trifolium pratense cv 'Minshan' isoflavone on performance and bone mineralization and blood profile of laying hens in later period

试验旨在研究岷山红三叶异黄酮对产蛋后期母鸡生产性能、骨矿化、血清生化指标、内分泌激素水平等的影响。将50周龄罗曼蛋鸡450羽,随机分成5组,每组6个重复,每个重复15羽。1组(对照)饲喂玉米-豆粕-杂粕型基础饲粮,2,3,4,5组分别在基础饲粮中添加5,10,20 mg/kg红三叶异黄酮和20 mg/kg大豆异黄酮(参比)。预试期2周,正试期10周。结果表明,1)各组生产性能和蛋品质无显著差异(P>0.05),呈现出添加红三叶异黄酮提高而添加大豆异黄酮(5组)降低蛋壳强度的趋势(P=0.213);2)4组脾脏指数高于1,3(P<0.05)和5组(P=0.150);3)4组胫骨灰含量高于1,2组(P<0.05),3,5高于2组(P=0.140,P=0.152);4)添加红三叶异黄酮各组血清钙含量提高(P=0.091),大豆异黄酮提高血清钙含量的作用似更强;5)4组血清甲状腺素(T4)含量高于1(P<0.05)和2,3组(P=0.150,P=0.20),5组高于1组(P=0.074)。结果提示,饲粮中添加5,10,20 mg/kg红三叶异黄酮和20 mg/kg大豆异黄酮对产蛋后期母鸡产蛋性能无显著影响;添加红三叶异黄酮一定程度上改善了蛋壳强度,添加量20 mg/kg促进了骨矿化。

The effects of Trifolium pratense cv ＇Minshan＇ isoflavone（TPI）on performance,bone mineralization,blood profil,e and endocrine hormone level of layers during the later laying period was studied.A total of 450,50-week-old Roman layers were divided randomly into five groups with six repeats of 15 layers each.These groups were fed basal diet composed of corn,soybean meal and flax meal,alone in group 1,or with a supplement of 5,10,or 20 mg/kg TPI,or with 20 mg/kg soy isflavone（SI）（groups 2,3,4,and 5 respectively）.After a preliminary period of two weeks,the trial lasted 10 weeks.1）There was no significant effect of treatments on the performance and egg quality（P0.05）,but there was a tendency for eggshells to be stronger in groups with TPI added to the diet but lower for the group of 20 mg/kg SI（P=0.213）.2） The spleen index was higher for group 4 than for group 1,3（P0.05）,and 5（P=0.150）.3） The content of tibia ash from group 4 was higher than that from groups 1 and 2（P0.05）,and the contents for groups 3 and 5 tended to be higher than that for group 2（P=0.140,P=0.152）.4） The serum calcium level was higher for groups with added TPI（P=0.091）,with the highest in group 5.5）Serum thyroxine（T4） content was higher for group 4 than for group1（P0.05） and for groups 2（P=0.150） and 3（P=0.20）,and that for T4 was also higher than group 5 and group 1（P=0.074）.No significant effects were observed on the production performance of the later laying period of hens as a result of adding 5,10,or 20 mg/kg TPI or 20 mg/kg SI,but adding TPI improved eggshell strength to a certain degree and adding 20 mg/kg（group 4） of TPI improved bone mineralization.