西南五省区及非洲野生狗牙根种质基于SRAP标记的遗传多样性分析

Genetic diversity of wild Cynodon dactylon germplasm from five province of Southwest China and Africa detected by SRAP markers

利用SRAP分子标记技术,对采自中国西南5省区的44份野生狗牙根及8份非洲狗牙根材料进行遗传多样性研究。结果表明,18对引物组合共得到扩增总条带236条,多态性条带数206条,平均每对引物扩增出11.4条带,多态性位点百分率为87.29%,材料间的遗传相似系数范围为0.569-0.929,平均GS值为0.723。聚类分析结果表明,52份材料可聚为5类;基于Shannon多样性指数估算了8个狗牙根生态地理类群内和类群间的遗传分化,类群内的遗传变异占总变异的63.81%,类群间的遗传变异占总变异的36.19%,表明这些抗源材料遗传差异较大,各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。

Sequence-related amplified polymorphism（SRAP） markers were used to detect the genetic diversity of 44 wild accessions of Cynodon dactylon collected from Sichuan,Chongqing,Yunnan,Guizhou and Tibet of China,and 8 wild accessions of C.dactylon collected from Africa.The results showed that eighteen primer pairs produced 206 polymorphic bands,averaged 11.4 bands per primer pair.The percentage of polymorphic bands in average was 87.29%.The Nei＇s genetic similarity coefficient of the tested accessions ranged from 0.569 to 0.929,and the average Nei＇s coefficient was 0.723.The 54 accessions were classified into five major groups： Ⅰ,Ⅱ,Ⅲ,Ⅳand Ⅴ by cluster analysis using UPGMA,which showed significant relationship with the origin regions of accessions.Genetic differentiation between and within eight eco-geographical groups of C.dactylon was estimated by Shannon＇s diversity index,which showed that 63.81% genetic variance existed within group,and 36.19% genetic variance was among groups,indicating rather large genetic distances among those germplasm,and there was a correlation between genetic differentiation and eco-geographical habits among the groups.