鸭茅杂交种的SSR分子标记鉴定及其遗传变异分析

Identification and genetic variation analysis of orchardgrass hybrids(Dactylis glomerata)by SSR molecular markers

以140个鸭茅杂交种单株及其亲本(01996、YA02-103)为材料,用SSR分子标记技术研究杂种与其亲本间扩增谱带的多态性,以甄别真假杂种。SSR标记在供试材料中的扩增带型表现为互补型、父本型及其他型。利用3对特异引物A01G20、A03N16、A03K22可快速准确地鉴定出杂交种111份。试验所用的100对SSR引物中有13对引物分别在杂种和双亲之间存在扩增多态性。13对引物在供试材料中共扩增出多态性条带76条,平均每对引物扩增出6条,多态性百分率为84.24%。供试材料具有丰富的遗传变异,利用SSR进行鸭茅杂种鉴定及遗传变异分析是可行的。

Hybrids（140） of orchardgrass（Dactylis glomerata）and their parental lines（01996,YA02-103） were tested by simple sequence repeats（SSR） markers.The amplification bands of hybrids showed complementary patterns of both parents,the male pattern and other patterns.Three complementary pattern primers： A01G20,A03N16,A03K22 could effectively and reliably detect 111 hybrids.A total of 76 polymorphic bands were detected by 13 primer pairs,with an average of 6 bands per primer The percentage of polymorphic bands was 84.24%.These results suggested that there was rich genetic variation among all orchardgrass hybrids tested and that the SSR technique has wide prospective use in hybrid identification and genetic analysis of orchardgrass.