摘要
利用Southenblot和DNA碱基序列分析技术,对正常犬c-yes原癌基因的分布规律及c-yescD-NA的结构进行了分析。结果表明,犬体细胞染色质中c-yes基因均一分布,c-yescDNA开放阅读框架有539个氨基酸组成,分子量为60,368Da,与人和鸡p61c-yes同源性分别为95.9%和90.4%。该产物具有src(SH)3,SH2同源区和酪氨酸激酶序列,该序列与人和鸡的同源性分别为98.8%和96.0%。证明犬c-yes基因产物类似于人和鸡p61c-yes。
Canine c yes proto oncegene in genomic DNAs from a cDNA library derived from a healthy canine spleen using a human c yes 1 probe. This proto oncogene was well conserved in clinically normal dogs. The nucleotide and amino acid sequences revealed that the canine yes gene contained an open reading frame consisting of 539 amino acids. Its product had a molecular mass of 60368 Dalton and showed 95.9 percent and 90.4 percent homology with human and chick p61 c yse , respectively. Moreover, the product had src homology region (SH) 3, SH 2 and tyrosine kinase domains showing 98.8 percent and 96.0 percent homology with these of human being and chickens, respectively. These findings indicate that the products of the canine c yes gene maybe not with receptor type tyrosine kinase activity as is the case in human and chick p61 c yes .
出处
《实验动物科学与管理》
1998年第3期1-5,共5页
Laboratory Animal Science & Administration
基金
国家自然基金