犬c-yes原癌基因

Restriction Patterns and Sequence of the Canine c yes Proto oncogene

利用Ｓｏｕｔｈｅｎｂｌｏｔ和ＤＮＡ碱基序列分析技术，对正常犬ｃ－ｙｅｓ原癌基因的分布规律及ｃ－ｙｅｓｃＤ－ＮＡ的结构进行了分析。结果表明，犬体细胞染色质中ｃ－ｙｅｓ基因均一分布，ｃ－ｙｅｓｃＤＮＡ开放阅读框架有５３９个氨基酸组成，分子量为６０，３６８Ｄａ，与人和鸡ｐ６１ｃ－ｙｅｓ同源性分别为９５．９％和９０．４％。该产物具有ｓｒｃ（ＳＨ）３，ＳＨ２同源区和酪氨酸激酶序列，该序列与人和鸡的同源性分别为９８．８％和９６．０％。证明犬ｃ－ｙｅｓ基因产物类似于人和鸡ｐ６１ｃ－ｙｅｓ。

Canine c yes proto oncegene in genomic DNAs from a cDNA library derived from a healthy canine spleen using a human c yes 1 probe. This proto oncogene was well conserved in clinically normal dogs. The nucleotide and amino acid sequences revealed that the canine yes gene contained an open reading frame consisting of 539 amino acids. Its product had a molecular mass of 60368 Dalton and showed 95.9 percent and 90.4 percent homology with human and chick p61 c yse , respectively. Moreover, the product had src homology region (SH) 3, SH 2 and tyrosine kinase domains showing 98.8 percent and 96.0 percent homology with these of human being and chickens, respectively. These findings indicate that the products of the canine c yes gene maybe not with receptor type tyrosine kinase activity as is the case in human and chick p61 c yes .