天花粉蛋白诱发白血病细胞K562凋亡的研究

STUDY ON TRICHOSANTHIN INDUCED APOPTOSIS OF LEUKEMIA K562 CELLS

天花粉蛋白(Trichosanthin,TCS),是一种从栝楼块根内提取的核糖体失活蛋白,具有流产、抗肿瘤和抗HIV等多种生物活性。本文利用FACS检测到天花粉蛋白可使K562白血病细胞产生明显的凋亡小峰、DNA区带电泳成典型的“梯状”条带,电镜检测可观察到明显的细胞凋亡形态。这些结果表明天花粉蛋白可以诱发K562白血病细胞产生凋亡。

Trichosanthin (TCS), an eukaryotic ribo-some-inactivating protein isolated from the root tuber of Trichosanthes plant, has various biological activities including abortion induction, anti-tumor, and anti-HIV. In this study, cultured human leukemia K562 cells treated with trichosan-thin were examined. Analysis of the cells by single laser flow cytometry showed the sub-G1 peak. DNA extracted from these cells formed a characteristic 'ladder' on agarose gel electrophoresis. Under electromicroscope, typical morphological changes of apoptosis were also observed. From all of these findings, we concluded that tri-chosanthin was able to induce apoptosis in K562 cells.