单双波长在生物活性测定中的比较研究

The contrast research on single and dual wavelength detection about bioactivity

目的:通过比较研究,确定酶标仪单双波长哪个更适于生物制品活性测定实验。方法:样品通过多次实验单双波长扫描,比较得到的实验结果,进行统计分析。结果:选用双波长可以排除其他因素对结果的影响,实验结果的标准差和离散系数均较低。结论:单双波长法更适于生物活性测定实验,提高结果的准确度。

Objective：To choose a suitble method of bioactivity detection by making a contrast research,either microplate reader single wavelength or dual wavelength. Methods：Make many experiments of single wavelength and dual wavelength,compared all results,then analyzed statistically. Results：Choose dual wavelength detection,suitable for bioactivity detection,less SD and CV%. Conclusion：Single and dual wavelength are more accuracy and reduce interference factors.