穿心莲内酯联合5-FU对人胃癌BGC-823细胞的体内外抑制作用

Synergistic Effect of Andrographolide and 5-fluorouracil on the Inhibition of BGC-823 Cell line of Human Gastric Cancer

目的:探讨穿心莲内酯联合5-Fu对人低分化胃癌细胞株BGC-823的协同抗肿瘤作用。方法:以人胃癌BGC-823细胞株为靶细胞,通过体外细胞培养和裸鼠移植瘤试验,采用MTT法检测、绘制效应-合用指数曲线、抑瘤率计算等方法。结果:穿心莲内酯在7.5～120μg/mL、5-FU在3.75～60μg/mL范围内均能抑制BGC-823细胞的生长,具有剂量-效应关系和时间-效应关系。两药联合作用24h、48h、72h时的中效抑制浓度分别为17.32、9.54、4.11μg/mL,显著小于单药应用组。72h效应-合用指数曲线表明两药在抑制效应≤0.97范围内可产生协同作用。穿心莲内酯联合5-FU对人BGC-823细胞裸鼠移植瘤的抑瘤率为(75.51±12.57)%,与单药组相比差异均有显著性。结论:穿心莲内酯、5-Fu能在体内外抑制人胃癌BGC-823细胞的生长,两药联合产生协同或相加作用。

Objective： To study the anti -proliferation effects of the Andrographolide with the 5 -fluorouracil（5 -Fu） on BGC -823 cell line of human gastric carcinoma. Methods： MTr assay were applied to determine cell inhibition in vitro. Median inhibitory concentrations （ IC50 ） of andrographolide with 5 - FU or used alone were calculated; Tumor in- hibition rate of xenografts in nude mice were measured in vivo. Results ： Andrographolide at the dose of 7.5 ~ 120μg/mL and 5 - Fu at the dose of 3.75 ~ 60μg/mL can both inhibit the proliferation of BGC - 823 cells with a concentration and time dependent manner. The cell inhibition rates and xenografts inhibition rates （75.51 ＋ 12.57 ）% was obviously in- creased when the two agents were combined used. Median inhibitory concentration （itS0）of andrographolido with 5 -FU at the time of 24h, 48h, 72h werel7. 32 , 9. 54 , 4. ll/~g/mL respectively, which were significantly lower than single groups. The eurve of combination index - effect relationship indicated a synergistic effect produce by andragrapholide and 5 -FU when the response rate was lower than 97%. Conclusion： Andrographohde and 5 - Fu can inhibit the growth of BGC - 823 cell line in vitro and in vivo. Combination use of the two drugs will play a synergistic effect in the activity of anti -gastrie cancer.