摘要
宇佐美曲霉Ｌ３３５是一株分泌高单位酸性蛋白酶的生产菌，本研究采用单因素搜索和正交试验，通过摇瓶发酵和２Ｌ搅拌罐发酵，对其发酵工艺进行优化。结果表明，较适宜的发酵培养基（ｇ／１００ｍＬ）为黄豆粉５．０、玉米粉１．２、鱼粉０．８、ＣａＣｌ２０．５、Ｎａ２ＨＰＯ４０．２、ＮＨ４Ｃｌ１．０、蚕蛹水解液１０．０；通气量控制２５ｈ前为１∶０．４ｖ／ｖ／ｍ、２５－５０ｈ１∶１．０ｖ／ｖ／ｍ、５０ｈ后１∶１．３ｖ／ｖ／ｍ；孢子接种，３１±１℃培养８８ｈ。在上述较优发酵条件下，酸性蛋白酶活力在６１００ｕ／ｍＬ以上，发酵单位居国内领先地位。
Asp. usamii L336 strain could produce high yield of acid protease. For optimizing its fermentation conditions, a single factor searching and orthogonal test were carried out in flask and 2L agitation fermentor. The experiment results showed that ezyme activity of acid protease was over 6100 u/mL under the optimum condition. This fermentation unit is in the lead level in China.
出处
《化学反应工程与工艺》
EI
CAS
CSCD
北大核心
1998年第4期382-387,共6页
Chemical Reaction Engineering and Technology
