番鸭A-FABP基因部分序列多态性与屠宰、肉质性状和血清甘油三酯含量关系

Association of the A-FABP gene polymorphism with the slaughter trait,meat quality trait and serum triglyceride in muscovy

根据家鸭A-FABPmRNA序列和鸡A-FABP基因组序列设计1对引物扩增番鸭A-FABP基因内含子1序列。同时根据扩增产物设计5对引物,利用PCR-SSCP对番鸭A-FABP基因部分进行多态性研究及多态性与部分屠宰性状、肉质性状、胸肌肌内脂肪含量和血清甘油三酯含量关系。结果显示,克隆序列包含番鸭A-FABP基因外显子1、2部分序列和完整的内含子1序列,与家鸭和鸡A-FABP基因内含子1分别有序列94.43%和72.95%的同源性;经SSCP检测,在引物P3扩增片段上发现2处碱基突变:分别为1074处T-G和1089处A-C突变,这2处突变产生3种基因型AA、BB和AB型,χ2检验显示番鸭群体符合Hardy-Weinberg平衡(P>0.05)。最小二乘分析显示,AA、AB型个体在胸肌率、肌肉pH值以及胸肌肌内脂肪含量均存在显著性差异(P<0.05)。结果表明,番鸭A-FABP基因内含子1多态性可能对个体生产性能产生重要的影响。

A pair of primers P-in1 were designed according to the sequences of adipocyte fatty acid-binding protein （A-FABP） gene mRNA in duck and A-FABP gene in chicken to amplify muscovy A-FABP intron-1,then 5 pairs of primers were designed according to the cloned gene.The SNPs were detected by technique of PCR-SSCP,confirmed by sequencing,then studied the association of polymorphisms of A-FABP gene with the slaughter trait,meat quality trait and content of intramuscular fat and serum triglyceride.The results indicated that the product of primer P-in1 included partial exon-1,exon-2 and complete intron-1 after cloned and sequenced and homologous comparison with muscovy and duck,chicken A-FABP gene,was 94.43% and 75.1%.T-G at 1 074 and A-C at 1 089 nucleotide mutation were found in the products of primer P3,which resulted in 3 genotypes and the results of Chi-Square test showed that the groups was fit with the Hardy-Weinberg equilibrium （P〉0.05）.The least square analysis showed that there was significant difference （P〈0.05） between AA and AB genotype birds in percentage of breast muscle,pH value and IMF.The results suggested that the polymorphisms of intron 1 of A-FABP gene have a certain effect on performance trait.