一株经鸭胚传递的鸭副黏病毒的分离鉴定及生物学特性

Biological identification of a paramyxovirus strain isolated from ducklings and duck-embryos

从表现疑似副黏病毒症状的1日龄雏鸭和死亡鸭胚中分离到1株病毒,经HA、HI和病毒中和反应鉴定为副黏病毒,命名为SDFCH株。按常规方法测得SDFCH株的生物学毒力指标MDT、ICPI和IVPI分别为56.6 h、1.78和2.59,鸭胚半数致死量LD50为10^-8.5,表明SDFCH株为副黏病毒强毒株。用分离病毒对11日龄鸭胚和7日龄雏鸭攻毒,孵化出的雏鸭和攻毒的雏鸭均出现明显的剖检变化。根据GenBank中NDV F基因序列设计合成了3对引物,通过RT-PCR扩增出了鸭源Ⅰ型禽副黏病毒（APMV-1）SDFCH株的F基因。与已发表副黏病毒F基因相关序列对比结果表明：SDFCH株与鸭、鹅源副黏病毒的同源性较高,核苷酸和氨基酸的同源性均在96.4%-98.0%之间,而与Lasota株同源性较低,核苷酸和氨基酸同源性仅为84.4%和87.9%。表明该毒株相对于传统的Lasota株已经发生了较大的变异。

A strain virus isolated from duckling and duck-embryos was identified as paramyxovirus by HA,HI and neutralization of virus.The virus was named SDFCH strain.The MDT,ICPI and IVPI for SDFCH strain were 56.6 h,1.78 and 2.59,respectively.LD50 of duck-embryos is 10^-8.5.The results indicated that SDFCH isolate was virulent strain.Ducklings and duck-embryos which were challenged by paramyxovirus SDFCH strain had visible pathological change.The fusion（F） gene of SDFCH strain was amplified by the RT-PCR using three pairs of primers designed according to the published gene sequences.The F gene of SDFCH strain exhibited 96.4%-98.0% nucleotide and 96.4%-98.0% amino acid homology to those of paramyxoviruses isolated from ducks and gooses.But only 84.4% nucleotide and 87.9% amino acid homology to Lasota strain.The results indicated that SDFCH strain was genetically closer to paramyxoviruses isolated from ducks and gooses than to Lasota strain.