赛拉唑对大鼠不同脑区NO-NOS-cGMP信号转导系统的影响

Influence of xylazole on NO-NOS-cGMP signal pathways in different brain regions in rats

观察赛拉唑对大鼠不同脑区NOS活性、NO和cGMP含量的影响,以探讨NO-NOS-cGMP信号转导系统对赛拉唑全麻分子机理的调控。Wistar纯种大鼠84只,随机选取12只为生理盐水对照组,其余随机均分为低剂量赛拉唑用药组和高剂量赛拉唑用药组,每个剂量组又分为麻醉期、翻正反射恢复期和苏醒期3个亚组(各12只)。用分光光度法测定大鼠不同脑区NOS活性和NO产量,放射免疫法测定脑cGMP含量。结果表明,赛拉唑能明显地抑制大鼠大脑皮质、小脑、海马和脑干NOS活性、NO和cGMP含量,并且NOS活性、NO含量的抑制作用呈现剂量依赖性增加趋势,这种变化与大鼠赛拉唑麻醉后行为学变化相吻合。结果提示,NO-NOS-cGMP信号传递系统参与了赛拉唑全麻作用产生的分子学机制的调控。

The effect of xylazole on the activity of NOS,and content of NO and cGMP in different brain regions of rats were observed to investigate the modulation of NO-NOS-cGMP signal transduction in the molecular mechanisms of general anaesthesia mediated by xylazole.Eighty and four Wistar ats were randomly divided into conrol group（12 rats）,high dose xylazole group（intraperitoneal xylazole 100 mg/kg） and low dose xylazole group（intraperitoneal xylazole 80 mg/kg）,with three sub-groups（eg anesthesia,recovery of righting reflex and aroused groups each）.The activity of NOS and content of NO were measured by spectrophotometric analysis.The cGMP content were measured by radioimmunoassay.The results indicated that xylazole significantly inhibited the activity of NOS,and reduced the content of NO and cGMP in cerebral cortex,hippocampus,cerebellum and brain stem in a dose-dependent manner.The inhibited activity of NOS and content of NO in cerebral cortex,hippocampus,cerebellum and brain stem tend to dose-response.The results indicated that NO-NOS-cGMP pathway in cerebral cortex,hippocampus,cerebellum and brain stem could be the targets of general anesthetic action mediated by Xylazole.