灵芝液体发酵富集硒元素研究

Selenium dosage for submerged culturing enriched Ganoderma lucidum

研究了灵芝在亚硒酸钠水平分别为50μg/mL、100μg/mL、150μg/mL、200μg/mL、250μg/mL、300μg/mL、350μg/mL、400μg/mL的固体PDA培养基上的生长情况,30℃恒温培养6d,观察并测定菌落直径;在不同亚硒酸钠浓度条件下进行液体发酵,180r/min转速下30℃摇床培养5d,检测发酵液生物量、富硒率,同时检测富硒后菌丝体部分金属元素的含量变化。结果表明:150μg/mL^250μg/mL的含硒固体PDA培养基上菌丝体生长好,在含硒250μg/mL液体发酵培养基上菌丝体生物量最高为0.86g,300μg/mL发酵液中富硒率最高为47.71%,而且富硒后的菌丝体其他金属元素含量变化不明显。

The growth conditions of Ganoderma lucidum on the level of the sodium nitrite at 50μg/ml, 100μg/ml, 150μg/ml, 200μg/ml, 250μg/ml, 300μg/ml, 350μg/ml, 400μg/ml of solid PDA medium were studied in this paper. After cultivation for 6 d at 30℃, the colony dimeter was, observed and measured. As well as in liquid fermentation medium selenium-enriched conditions, 180 r/min under 30 ℃ for 5 d. The biomass and fermentation rate of selenium,were detected and the results showed that： 150-250μg/ml of Se-riched solid PDA medium hypha growth is good, Se 250μg/ml in liquid fermentation medium, mycelium biomass up to 0.86 g, 300μg/ml broth Se-highest rate of47.71%. And the other metal element content of the selenium-enriched mycelia does not change significantly.