滋阴清热方对MRL/lpr狼疮鼠皮质醇及糖皮质激素受体mRNA表达的影响

Effects of Nourishing Yin and Clearing Heat Decoction on Level of Glucocorticoid and the Glucocorticoid Receptor mRNA Expression in MRL/lpr Mice

目的观察MRL/lpr狼疮鼠皮质醇及糖皮质激素受体(GR)mRNA表达的变化及滋阴清热方对其水平的影响,探讨中药减轻激素不良反应的机理。方法 40只MRL/lpr狼疮模型鼠随机分为4组,即模型组、中药组、西药组、中西药组。分别给予生理盐水、滋阴清热方、强的松悬液、滋阴清热复方+强的松悬液灌胃,均每日1次,每次0.5mL,连续用药10周。另选用昆明小鼠10只作为正常对照组。应用放射免疫法测定MRL/lpr狼疮鼠糖皮质醇水平,反转录酶-聚合酶链反应法(RT-PCR)检测GR mRNA,观察各组皮质醇、GR mRNA表达的变化。结果模型组血清皮质醇水平与正常组比较,P>0.05,而GRα mRNA表达明显低于正常组(P<0.05)。西药组GRα mRNA、GRβmRNA下调水平高于中药组和中西药组(P<0.01或P<0.05)。结论滋阴清热方可上调MRL/lpr狼疮鼠GR mRNA表达水平,从而增强糖皮质激素的临床疗效,减少激素不良反应。

Objective To observe the change of glucocxnticoid （GC） and the messenger RNA expression of its receptor （GRmRNA） in MRL/1pr mice with the intervention of nourishing yin and clearing heat decoction. Methods Forty MRL/1pr mice were randomly divided into four groups; model control group, TCM group, west medicine group, combined treatment of traditional chinese medicine and west medicine group, 10 mice in each group. The group was intragastrically administrated normal sodium, nourishing yin and clearing heat decoction, prednisone suspension, nourishing yin and clearing heat decoction plus prednisone suspension, 0.5 mL every time, once daily for 10 weeks respectively. Meanwhile, 10 Kungrrdng mice were selected as normal group, administrated normal sodium, like the model group. The expression of glucocxnticoid receptor-alpha and beta （GR a mRNA, GR 13 mRNA） were detected by reverse transcription polymerase chain reaction （RT-PCR）. Results Compared with normal, the expression of GRmRNA was increased in model groups, but the level of GC was not different. Compared with model group, GR a mRNA and GR 13 mRNA of MRL/Ipr mice were improved in all treatment groups, especially in combined treatment of traditional Chinese medicine and western medicine group. Conclusion Nourishing yin and clearing heat decoction has effect of upregulating the expression of GRmRNA in MRL/1pr mice, so as to enhance the effect of glucocorticoid and reduce its side effects.