高效液相色谱-串联质谱法测定食品中20种氨基甲酸酯类农药残留

Determination of 20 carbamate pesticide residues in food by high performance liquid chromatography-tandem mass spectrometry

建立了食品中20种氨基甲酸酯类农药残留量的高效液相色谱-串联质谱联用(HPLC-MS/MS)测定与确证方法。20种氨基甲酸酯类农药在0.005～0.1mg/kg范围内线性良好,相关系数为0.9917～0.9996;在0.005～0.025mg/kg范围内,20种目标物的回收率为51.2%～125.0%,相对标准偏差为1.4%～19.8%。该方法准确、灵敏、快速,可满足国际上对食品中这20种氨基甲酸酯类农药残留量的检测需要。

A new method of determination and conformation for 20 carbamate pesticide residues in food by high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） was developed.The residual carbamate pesticides in food were extracted with acetonitrile,cleaned up with C18 solid phase extraction column or Carb/NH2 solid phase extraction column,and the targets were detected and confirmed by HPLC-MS/MS using external standard method.The recoveries of the 20 carbamate pesticides in spiked levels （from 0.005 to 0.025 mg/kg） ranged from 51.2% to 125.0% with the relative standard deviations （RSDs） from 1.4% to 19.8%;the concentration range of linearity was 0.005-0.1 mg/kg;the correlation coefficients were 0.991 7-0.999 6.The method is fast,easy,sensitive and reliable.It also demonstrates that this method can meet the requirements for the simultaneous determination of 20 carbamate pesticide residues in food.