胶束电动毛细管色谱法检测红曲米中的莫纳可林K

Determination of Monacolin K in red fermented rice by micellar electrokinetic capillary chromatography

建立了测定红曲米中莫纳可林K含量的胶束电动毛细管色谱(MEKC)方法。考察了运行缓冲液的种类、pH及其浓度、有机添加剂、十二烷基硫酸钠(SDS)的浓度和分离电压等实验条件对电泳分离效果及检测灵敏度的影响。在优化的实验条件下,以20mmol/L硼砂(pH10.6,含10%(体积分数)乙醇和40mmol/LSDS)作为缓冲溶液,莫纳可林K能在23min内实现很好的基线分离,线性范围为5.00～100.00mg/L,线性相关系数为0.9976,检出限(以信噪比(S/N)为3计)为0.13mg/L,加标回收率为98.5%～99.5%。精密度和稳定性试验中,峰面积和迁移时间的相对标准偏差均小于3%,表明重复性良好。该方法简便、快速、灵敏,可用于红曲米中莫纳可林K含量的测定。

A method for Monacolin K determination in red fermented rice based on micellar electrokinetic capillary chromatography has been developed.The assay conditions including pH and the concentration of running buffer,organic additive,the concentration of sodium dodecyl sulfate （SDS）,and the separation voltage were optimized.Under the optimized conditions （20 mmol/L borate buffer （pH 10.6,containing 10%（v/v） methanol,40 mmol/L SDS）,the Monacolin K can be separated within 23 min,with the linear working range of 5.00-100.00 mg/L （r2=0.9976） and a limit of detection （S/N=3） of 0.13 mg/L.It had good recoveries （98.5%-99.5%） and the relative standard deviations lower than 3%.The method is simple,rapid,sensitive,highly reproducible and can be successfully applied in the determination of Monacolin K in red fermented rice.