不同热剂量对PLC细胞株杀伤效应及抗肿瘤免疫效应比较研究

The Comparative Study for PLC Cell Strain Lethal Effect And Anti-Tumor Immunological Effect by Different Heat Dose

目的探讨不同热剂量对PLC细胞株的杀伤效应及机体免疫效应,为本地区高发肺癌临床开展热疗提供初步实验依据。方法体外实验采用MTT法检测不同热剂量对PLC杀伤率,得出适宜的杀伤热剂量,为体内试验提供依据。体内试验选取45℃与55℃作为实验组,进行肿瘤体积测量、T细胞CD4及CD8亚群检测、IL-2浓度监测。结果体外实验结果表明于44-46℃及54-56℃区间出现较强细胞杀伤,且联合DDP组呈现更强杀伤效应;体内实验结果表明45℃组与55℃组均较对照组在抑瘤效应及免疫效应具有显著差异,尤以55℃组为甚。结论热消融温度（55℃）在较短时间内杀伤肿瘤细胞,呈现出抑瘤明显且免疫效应明显等特点,值得临床参考。

OBJECTIVE：To approach PLC cell strain lethal effect and anti-tumor immunological effect by different heat dose,in order to supply experiment evidence for native lung cancer carry out thermotherapy.METHOD：Extraorgan experiment was adopt MTT to detect lethal effect by different heat dose,so that to obtain suitable heat dose in order to vivo experiment.Vivo experiment was adopt 45℃ and 55℃ experimental group,to execute measure tumor volume、detect CD4 and CD8 T-cells、monitor IL-2 concentration.RESULTS： The result of extraorgan experiment that 44~46℃ and 54~56℃ interval had the stronger lethal effect,and combine DDP was better than another experiment group.The result of vivo experiment that 45℃ group and 55℃ group have better lethal effect and anti-tumor immunological effect than control group,above all 55℃ group.CONCLUSION：The ablation temperature（55℃） could damage tumor cells in shorter time,and have stronger anti-tumor immunological effect,to be worth clinical reference.