微量元素对转植酸酶基因小球藻生长的影响

Effect of Micro-Elements on Growth of Transgenic Marine Chlorella sp. MACC/C95

研究了锌、钼、钴、铜、锰、铁6种微量元素对转植酸酶基因小球藻生长的影响.通过单因子实验确定了此6种微量元素促进转基因小球藻生长及胞内植酸酶表达的浓度范围,通过正交实验确定了锌、钼、钴、铜、锰促进转植酸酶基因小球藻生长的最佳浓度,获转植酸酶基因小球藻最大生物量产量的浓度依次为0.10、0.015、0.01、0.015、0.8μmol/L;获转植酸酶基因小球藻最高植酸酶比活值的浓度依次为0.10、0、0.04、0.015、0.4μmol/L.利用微量元素优化配方培养转植酸酶基因小球藻,可使胞内植酸酶比活值显著提高,但微藻生物量产量有所下降.

The effects of mico-elements, such as zinc, molybdenum, cobalt, copper, manganese, and iron in the cultural liquid on the growth of transgenic marine ChloreUa sp. MACC/C95 are studied in batch culture. Their beneficial concentrations are defined by single factor experiments. The results of orthogonal experiments show that the optimal concentrations on the growth of C. sp in cultural liquid are Zn^2＋ 0. 10 μmol/L, Mo^2＋ 0. 015 μmol/L, Co^2＋ 0. 01 μmol/L, Cu^2＋ 0. 015 μmol/L, Mn^2＋ 0. 8 μmol/L, and the optimal concentrations on phytase content of C. sp in cultural liquid are Zn^2＋ 0. 10 μmol/L, Mo^2＋ 0 μmoL/L, Co^2＋ 0. 04 μmol/L, Cu^2＋ 0. 015 μmol/L, Mn^2＋ 0. 4 μmol/L. When micro-elements of the optimal concentrations are added in the cultural liquid, the phytase content of C. sp is significantly increased, but the biomass is decreased.