摘要
目的:探讨早期糖尿病大鼠肾组织中尾加压素Ⅱ(urotensin Ⅱ,UⅡ)的表达及其意义。方法:用链脲佐菌素(STZ)诱导糖尿病大鼠模型,将实验动物分为正常对照组(C组)、糖尿病组(D组)和胰岛素干预组(Y组)。5周后收集肾组织,观察各组肾脏病理情况及UⅡ在肾组织中的表达。结果:处理5周后,与C、Y组比较,D组肾小球肥大、肾小球表面积显著增大,小管扩张,上皮细胞肿胀;D组肾小管上皮细胞UⅡ表达明显升高(与C组比较,P<0.05);Y组肾小管上皮细胞UⅡ表达有不同程度的下降(与D组比较,P<0.05)。结论:STZ诱导的早期糖尿病大鼠肾小管上皮细胞UⅡ表达明显上调;应用胰岛素严格控制血糖可部分下调肾组织UⅡ的表达。UⅡ在STZ诱导的早期糖尿病大鼠肾脏病变的发展过程中可能起一定作用。
Objective： To explore expression of urotensin Ⅱ in renal tissue in STZ-rats with diabetes and its significance. Methods： Diabetic rats were induced by streptozocin（STZ）, the experimental rats were randomly divided into three groups： normal control group（group C）, diabetic rats（group D） and diabetic rats treated by insulin（group Y）. 5 weeks later, the kidney specimens in different group were randomly collected and fixed in formalin and embedded with paraffin. The expression level and distribution of urotensin Ⅱ were evaluated with immunohistochemistry technique. Results： After treatment of 5 weeks, urotensin Ⅱ levels in renal tubule epithelial cells in group D increased remarkably compared with those in group C（P0.05）,while UⅡ levels renal glomerulus endothelial cells in group D didn′t increase markedly.In comparison with group D,urotensinⅡ levels were significantly reduced in group Y （P0.05）. Conclusion： The expression of UⅡ in renal tubule epithelial cells upregulated markedly in STZ-rats with diabetes;rigid controls of blood glucose by insulin can partly downregulate the expression of UⅡin renal tissue.UrotensinⅡmight play a role in the development of renal pathological changes in STZ-rats with diabetes.
出处
《中国当代医药》
2010年第9期19-21,共3页
China Modern Medicine
