溶剂抽提、水蒸气蒸馏和超临界萃取用于古蓬香脂组成分析方法的比较(英文)

COMPARISON OF SOLVENT EXTRACTION, STEAM DISTILLATION AND SUPERCRITICAL FLUID EXTRACTION OF GALBANUM

采用乙醇和丙酮溶剂抽提、ＣＯ２超临界萃取（ＳＦＥ）以及水蒸气蒸馏（ＳＤ）的方法提取古蓬香脂乳胶。对乙醇和丙酮提取物进行了ＨＰＬＣ和ＧＣ－ＭＳ分析。两个提取物的ＨＰＬＣ谱图十分相似，总共可分离出１７个组分。而乙醇提取物的ＧＣ－ＭＳ分析鉴定表明有２１个组分。ＳＦＥ提取物的ＧＣ－ＭＳ分析得到８个组分。ＳＤ提取物有１４个组分。在超临界流体中加入甲醇无论对提取物的组分数还是对其浓度都没有什么影响。

Galbanum latex was extracted by ethanol, acetone, supercritical carbon dioxide and steam distillation . The ethanol and acetone extracts were analyzed by HPLC as well as GC MS. The HPLC chromatograms of the acetone and ethanol extracts were very similar and a total of seventeen consitituents were observed, whereas the GC MS analysis of ethanol extract results in the observance of twenty one constituents. The GC MS analysis of SFE and SD revealed only eight and fourteen constituents respectively. Addition of methanol, to the supercritical fluid, had no effect on either the number or the concentratin of the constituents of SFE extracts.