高效液相色谱法测定环境水中痕量4,4′-二氨基联苯、4-硝基酚和苯酚

Determination of Trace 4,4′ Diaminobiphenyl, 4 Nitrophenol and Phenol in Environmental Water by High Performance Liquid Chromatography

采用ＰｈｅｎｏｍｅｎｅｘＳｐｈｅｒｅｘＣ１８色谱柱，以含５０ｍｍｏｌ／Ｌ乙酸－乙酸钠缓冲溶液（ｐＨ６．０）的Ｖ（乙腈）∶Ｖ（乙醚）∶Ｖ（水）＝１２∶１０∶７８混合溶液为流动相，用分光光度法检测，检测波长为２７５ｎｍ，在１６ｍｉｎ内实现了４，４′－二氨基联苯、４－硝基酚和苯酚的同时分离测定。检测限分别为０．１４，０．１９和０．０８ｎｇ。标准回收率分别为９８．８５％，９８．１５％和９８．１０％。方法灵敏度高，用于环境水样分析时结果令人满意。

A method for simultaneous determination of trace 4,4′ diaminobiphenyl, 4 nitrophenol and phenol in waste water by high performance liquid chromatography with spectrophotometric detection is reported. These components were separated on a Phenomenex Spherex C 18 column with V( acetonitrile )∶ V( ether ) ∶V( 50 mmol·L -1 acetate) =12∶10∶78 buffer (pH 6.0) as mobile phase and UV detection at 275 nm. The detection limits were 0.14, 0.19 and 0.08 ng for 4,4′ diaminobiphenyl, 4 nitrophenol and phenol, respectively, when the ratio of signal to noise was 2. The relative standard deviation was<2 2(n=6) This method is sensitive and has been applied to the analysis of environmental water samples with satisfactory results.