摘要
采用PhenomenexSpherexC18色谱柱,以含50mmol/L乙酸-乙酸钠缓冲溶液(pH6.0)的V(乙腈)∶V(乙醚)∶V(水)=12∶10∶78混合溶液为流动相,用分光光度法检测,检测波长为275nm,在16min内实现了4,4′-二氨基联苯、4-硝基酚和苯酚的同时分离测定。检测限分别为0.14,0.19和0.08ng。标准回收率分别为98.85%,98.15%和98.10%。方法灵敏度高,用于环境水样分析时结果令人满意。
A method for simultaneous determination of trace 4,4′ diaminobiphenyl, 4 nitrophenol and phenol in waste water by high performance liquid chromatography with spectrophotometric detection is reported. These components were separated on a Phenomenex Spherex C 18 column with V( acetonitrile )∶ V( ether ) ∶V( 50 mmol·L -1 acetate) =12∶10∶78 buffer (pH 6.0) as mobile phase and UV detection at 275 nm. The detection limits were 0.14, 0.19 and 0.08 ng for 4,4′ diaminobiphenyl, 4 nitrophenol and phenol, respectively, when the ratio of signal to noise was 2. The relative standard deviation was<2 2(n=6) This method is sensitive and has been applied to the analysis of environmental water samples with satisfactory results.
出处
《色谱》
CAS
CSCD
北大核心
1998年第6期536-538,共3页
Chinese Journal of Chromatography
关键词
二氨基联苯
硝基酚
苯酚
分析
水质监测
HPLC
high performance liquid chromatography, 4,4′ diaminobiphenyl, 4 nitrophenol, phenol