摘要
为了提高转萝卜过氧化物酶基因RsPrx1毕赤酵母株系的表达产量,对培养条件进行优化。试验结果表明,26℃,pH7.4,甲醇浓度0.5%,通气面积1.5cm2,225r/min条件下,萝卜过氧化物酶RsPrx1表达量达50.95U/mL。在优化条件下,对培养基补充方式和菌株的遗传稳定性研究表明,彻底更换培养基较补充培养基提高3倍得率,转基因菌株遗传稳定性较高。
The optimum cultivation have been investigated for improved heterologous expression of RsPrx1(a peroxidase of radish)in Pichia pastoris.The results showed that the expression of RsPrx1 could reached 50.95 U/mL in 26℃,pH7.4,0.5% methanol,1.5 cm^2 aeration area,225 r/min.In the optimum cultivation,the total activity of peroxidase was about 3 times higher in absolutely replaced medium continuous culture than that partly added medium continuous culture.The PCR results showed the transgenic strain was hereditary stability.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第4期203-206,共4页
Biotechnology Bulletin
基金
国家自然科学基金项目(30570135)
国家大学生创新性实验计划项目(081047609)
河南师范大学大学生创新性实验计划项目(2008405)
河南师范大学(国家级)实验教学示范中心大学生创新性实验计划项目