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转萝卜过氧化物酶基因毕赤酵母株系培养条件的优化 被引量：1

The Optimum Cultivation for Improved Heterologous Expression of Radish Peroxidase in Pichia pastoris
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摘要 为了提高转萝卜过氧化物酶基因RsPrx1毕赤酵母株系的表达产量,对培养条件进行优化。试验结果表明,26℃,pH7.4,甲醇浓度0.5%,通气面积1.5cm2,225r/min条件下,萝卜过氧化物酶RsPrx1表达量达50.95U/mL。在优化条件下,对培养基补充方式和菌株的遗传稳定性研究表明,彻底更换培养基较补充培养基提高3倍得率,转基因菌株遗传稳定性较高。 The optimum cultivation have been investigated for improved heterologous expression of RsPrx1（a peroxidase of radish）in Pichia pastoris.The results showed that the expression of RsPrx1 could reached 50.95 U/mL in 26℃,pH7.4,0.5% methanol,1.5 cm^2 aeration area,225 r/min.In the optimum cultivation,the total activity of peroxidase was about 3 times higher in absolutely replaced medium continuous culture than that partly added medium continuous culture.The PCR results showed the transgenic strain was hereditary stability.
作者 王一帆 李芳军 杨静 王丽 王林嵩
机构地区 河南师范大学生命科学学院
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第4期203-206,共4页 Biotechnology Bulletin
基金 国家自然科学基金项目(30570135) 国家大学生创新性实验计划项目(081047609) 河南师范大学大学生创新性实验计划项目(2008405) 河南师范大学(国家级)实验教学示范中心大学生创新性实验计划项目
关键词 萝卜过氧化物酶 毕赤酵母 转基因 培养条件 Radish peroxidase Pichia pastoris Transgene Culture condition
分类号 TQ925 [轻工技术与工程—发酵工程]
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参考文献13

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二级参考文献16

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生物技术通报
2010年 第4期

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