功能聚间苯二胺膜用于免疫传感器分析

Application of Functional Electropolymerized mPhenylene Diamine Membrane in Immunosensor Analysis

以电化学聚合法在石墨电极上获得聚间苯二胺膜（ＰＭＰＤ），并建立了快速、灵敏的膜质量检验方法：辣根过氧化物酶（ＨＲＰ）标记ＰＭＰＤ膜－邻苯二胺（ＯＰＤ）反应法，对聚合、活化等条件作了研究；进一步制成了检测乙肝表面抗原（ＨＢｓＡｇ）和检测鼠免疫蛋白（ＭＩｇＧ）的免疫电极，对血清样品作了检测．结果表明：２．５Ｖ电池电压下，于含０．１２ｍｏｌ／ＬＭＰＤ的１．２ｍｏｌ／ＬＨ２ＳＯ４中聚合２０ｍｉｎ，再以３０ｇ／Ｌ浓度的戊二醛活化４ｈ，所得ＨＲＰ标记电极及免疫电极重现性好；检测ＨＢ－ｓＡｇ和ＭＩｇＧ的线性范围分别为０．１～３．２ｍｇ／Ｌ及０．

An electropolymerization method of mphenylene diamine membrane, polyphenylene diamine(PMPD) on the surface of graphite electrode is described. To identify the quality of the membrane, a rapid and sensitive method has been established based on the reaction of a horseradish peroxidase(HRP) labeled PMPD with orthophenylene diamine. An antiHBsAg and an antiMIgG immunoelectrodes were prepared for detecting hepatitis B surface antigen(HBsAg) and mouse immunoglobin G(MIgG). The optimum membrane preparation conditions are polymerization: 012 mol/L MPD solution in 12 mol/L H2SO4 for 20 min under 25 V, activation: with 3% glutiraldehyde for 4 h. The prepared HRP labeled electrode and immunoelectrodes possessed good repetition. Catibration graphs were linear for HBsAg in ranges from 0132 g/mL and 0120 g/mL for MIgG/mL.