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循环DNA p16基因启动子区甲基化检测在非小细胞肺癌早期诊断中的意义

The significance of promoter hypermethylation of p16 gene in circulating DNA on early diagnosis of non-small cell lung cancer.
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摘要 目的评价检测循环DNAP16基因启动子区异常甲基化对非小细胞肺癌(NSCLC)早期诊断的价值。方法应用甲基化特异性PeR方法检测NSCLC患者及NSCLC高危人群循环DNAP16基因启动子区甲基化频率,评价其诊断价值。结果NSCLC患者循环DNAP16基因甲基化阳性率286%(6/21),高于非吸烟健康组0%(0/15,P=0.030),而NSCLC患者与重度吸烟组(10%,2/20),以及重度吸烟组与非吸烟健康组间差异均不具有统计学意义(P=0238,P=0496)。结论检测循环DNAP16基因启动子区异常甲基化,有助于对NSCLC高危人群的筛查及对NSCLC的早期诊断。 Objective To evaluate the diagnostic value of detecting promoter hypermethylation of p16 gene in circulating DNA from non-small cell lung cancer (NSCLC) patients. Methods Methylation-specific PCR (MSP) was performed for the detection of promoter hypermethylation of p16 gene in circulating DNA from NSCLC patients and from high-risk groups of NSCLC. Its clinical diagnostic value of NSCLC was evaluated. Results The detectable rate of promoter hypermethylation of p16 gene in circulating DNA from NSCLC patients was 28.6% (6/21), higher than that of the non- smoking healthy group (0%, 0/15,P=0.030), while there was no significant difference between the detectable rate from NSCLC patients and that from the heavy smoking group (10%, 2/20, P=0,238), as well as that from the heavy smoking group and that from the non-smoking healthy group (P=0.496). Conclusion Detecting promoter hypermethylation of p16 gene in circulating DNA may be helpful screening for NSCLC in high-risk population, and for the early diagnosis of NSCLC.
出处 《中国保健营养(下半月)》 2010年第4期21-23,共3页 China Health Care & Nutrition
基金 山东省博土基金项目(2004BS2005)子课题
关键词 非小细胞肺癌 循环DNA P16基因 DNA甲基化 NSCLC, circulating DNA, p16 gene, DNA hypermethylation
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参考文献14

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