摘要
目的评价检测循环DNAP16基因启动子区异常甲基化对非小细胞肺癌（NSCLC）早期诊断的价值。方法应用甲基化特异性PeR方法检测NSCLC患者及NSCLC高危人群循环DNAP16基因启动子区甲基化频率，评价其诊断价值。结果NSCLC患者循环DNAP16基因甲基化阳性率286％（6／21），高于非吸烟健康组0％（0／15，P=0．030），而NSCLC患者与重度吸烟组（10％，2／20），以及重度吸烟组与非吸烟健康组间差异均不具有统计学意义（P=0238，P=0496）。结论检测循环DNAP16基因启动子区异常甲基化，有助于对NSCLC高危人群的筛查及对NSCLC的早期诊断。
Objective To evaluate the diagnostic value of detecting promoter hypermethylation of p16 gene in circulating DNA from non-small cell lung cancer （NSCLC） patients. Methods Methylation-specific PCR （MSP） was performed for the detection of promoter hypermethylation of p16 gene in circulating DNA from NSCLC patients and from high-risk groups of NSCLC. Its clinical diagnostic value of NSCLC was evaluated. Results The detectable rate of promoter hypermethylation of p16 gene in circulating DNA from NSCLC patients was 28.6% （6/21）, higher than that of the non- smoking healthy group （0%, 0/15,P=0.030）, while there was no significant difference between the detectable rate from NSCLC patients and that from the heavy smoking group （10%, 2/20, P=0,238）, as well as that from the heavy smoking group and that from the non-smoking healthy group （P=0.496）. Conclusion Detecting promoter hypermethylation of p16 gene in circulating DNA may be helpful screening for NSCLC in high-risk population, and for the early diagnosis of NSCLC.
基金
山东省博土基金项目（2004BS2005）子课题
