荧光法研究Ge-132对牛血清白蛋白Mailard反应的抑制作用

The Suppression of Ge 132 on the Maillard Reaction of BSA by Fluorescence Spcectrum

人体内氨基酸及蛋白质的非酶糖化反应（Ｍａｉｌａｒｄ反应）与糖尿病及其并发症的关系已经引起广泛注意［１］．有机锗化合物β－羧乙基锗倍半氧化物（Ｇｅ－１３２）具有预防糖尿病及调节糖代谢的作用［２］．研究Ｇｅ－１３２对Ｍａｉｌａｒｄ反应的抑制作用，对于开发...

The fluorescences of BSA and glycosylated BSA were observed respectively. The λ em of BSA was 340 nm; while the λ em of glycosylated BSA was 436 nm. Because the fluorescence spectra of them were different greatly, we can observe the suppression of Ge 132 on the Maillard reaction of BSA without any interference of itself. It was showed that the fluorescence intensity of glycosylated BSA increased continuously with the cultured time, Ge 132 may suppress the Maillard reaction of BSA greatly, and the suppressing efficicency would be 32%. The key site of the Maillard reaction of BSA is free amino groups of alanine residues on N terminal amino group, besides the ε amino groups of intrachain Lysine residues.