牛肾组织及其培养物的不同消化法效果评价

Efficiency evaluation of the calf kidney tissue and culture digested with the different digestive fluid and method

为了比较不同消化液及消化方式对牛肾皮质组织和培养后形成单层细胞的消化分散效果并确定最适消化液和消化方式,分别用0.25%胰蛋白酶和0.25%胰蛋白酶-0.02%EDTA两种消化液,消化牛肾皮质组织及其培养形成的单层牛肾皮质细胞。牛肾皮质组织采用热(37℃)和冷(4℃)两种消化方式;经培养形成单层的牛肾皮质细胞采用室温(25℃)消化。结果显示,用0.25%胰蛋白酶-0.02%EDTA消化液热消化牛肾组织时,分散获得牛肾皮质细胞的活细胞数、存活率、贴壁率均优于其他消化方法,差异显著(P<0.05)。培养形成的单层牛肾皮质细胞用0.25%胰蛋白酶-0.02%EDTA消化液的消化速度明显快于用单一0.25%胰蛋白酶消化液的消化速度,统计学分析显示具有显著性差异(P<0.01),两种消化液消化所得细胞的存活率及贴壁率前者要更高,差异显著(P<0.05)。

In order to evaluate the digestion effect of the calf kidney cortex tissue and cultured cell monolayer with different digestive fluids and methods.The calf kidney cortex tissue was digested at 37℃,4℃ and room temperature（25℃）,respectively using 0.25% trypsase and 0.25% trypsase containing 0.02% EDTA.The results indicated that when digested at 37℃,the number of live cell,survival rate and attachment efficiency in the group of 0.25% trypsase containing 0.02% EDTA are higher than those of other groups（P〈0.05）.The digestion speed of the cultured calf kidney monolayer cell using 0.25% trypsase containing 0.02% EDTA is higher than that of other group（P〈0.01）.The survival rate and attachment efficiency of the digested cell in the two groups are different（P〈0.05）.