支原体培养基的改良及其效果评价

The improvement of Mycoplasma medium and evaluation

为了改良支原体培养基配方,评价其效果。按《中华人民共和国药典》(以下简称药典)中规定的灵敏度比较方法,将实验室制备的新型支原体改良培养基与《药典》中支原体检查法推荐的处方培养基和商品化支原体培养基进行灵敏度效果比较试验。结果表明,改良后的支原体肉汤和半流体培养基,与接种口腔支原体和肺炎支原体及其它支原体的精氨酸培养基、支原体肉汤培养基无显著差异;与接种肺炎支原体的支原体半流体培养基无显著差异;与接种口腔支原体的支原体半流体培养基差异显著。因此经改良后的支原体培养基灵敏度能够满足药典规定的要求,但其操作简便,且成本低于现有的支原体培养基。

According to the sensitivity comparison method described in the ＂The People＇s Republic of China Pharmacopoeia＂（hereinafter refer as CP）,the sensitivity comparison test will be conducted among the new mycoplasma media prepared in this laboratory,recommended media by mycoplasma detection in CP,and commercialized mycoplasma media.The results showed that there is no significant different between the new improved mycoplasma broth and semiliquid media,and other mycoplasma arginine media and mycoplasma broth media when inoculated with MO and MP,there is no significant difference between the new media and mycoplasma semiliquid media when inoculated with pneumoniae.There is no significant difference between them when inoculated with oral mycoplasma.Therefore the sensitivity of the improved mycoplasma media can meet the requirements and be easy to operate,and the cost lower than that of the existing mycoplasma media.