立体定向技术建立大鼠脑胶质瘤激光间质热疗模型

Applying stereotactic technique to establish C6 brain glioma models for laser interstitial thermotherapy research

目的利用立体定向技术接种SD大鼠C6脑胶质瘤，并建立脑胶质瘤激光间质热疗（LITT）模型。方法采用立体定向技术，将体外培养并调制的C6胶质瘤细胞悬液20μl（浓度1×10^11／L）接种于SD大鼠右侧尾状核区。分时段MRI检查；做组织病理学和Ⅷ因子相关抗原（FⅧR），胶质纤维酸性蛋白（GFAP）和S-100蛋白免疫组化检查。根据MRI扫描监测，校正肿瘤定位，按2～10W不同功率和热疗时间分组，插入半导体激光光纤进行间质热疗，同时使用ThermaCAM S65型红外热像仪测量肿瘤的中心靶点皮层温度和（或）热电偶仪间质测量靶区周边的深部温度。结果优化的立体定向接种技术使本组大鼠脑胶质瘤动物模型具有颅内生长稳定，成瘤率高，未见颅外转移病灶，实验周期短，可重复性好，可插入热疗光纤热疗，组织学上接近人类特征。LITT各组靶区温度高于假手术组（P〈0．05）；在同一治疗组内中心靶点皮层温度和靶区周边的深部温度之间有近似性，差异无统计学意义（P〉0．05）。结论利用立体定向技术可成功建立SD大鼠脑尾状核C6胶质瘤模型，其肿瘤MRI影像及病理特征与人脑胶质瘤相似。红外热像测温技术在大鼠实验性LITT研究中的应用具有可行性，可联合热电偶深部测温技术应用于脑肿瘤LITT治疗。

Objective C6 brain glioma models were established with stereotactic technique to study laser interstitial thermotherapy （LITT） in SD rats C6 intracranial glioma models. Methods The C6 cells cultured in vitro were stereotaxically implanted into the right eaudate nucleus of SD rat brain （20 μl free serum DMEM for one rat which concentration was 1 × 10^11/L）. The following step was to judge MRI scan. Tumor was confirmed with staining of ⅧR, GFAP and S- 100 immunohistochemistry. After MRI scanning and correction of tumor location, the models were divided into groups according treating time and laser power from 2 to 10 W. Semiconductor laser optical fibers were inserted in tumors for LITT, simultaneously cortex＇s temperature conducted from center target was measured by ThermaCAM S65 type infrared thermograph, and （or） deep tissue＇s temperature around target was measured by thermocouple. Results Inoculated with optimized stereotactic technique, rat C6 gliomas resembled histopathological features of human glioma. This kind of model was a more reliant and reproducible one, with 96. 67% yield of intracranial tumor as well as no extracranial growth extension. The difference between cortex temperature conducted from center target and deep tissue temperature around target had no statistical significance （ P 〉 0.05 ）. Conclusion A rat C6 brain glioma model resembles histopathological features of human glioma, as a perfect model to study LITT of glioma. Infrared thermograph technique to measure temperature conveniently, effectually, non invasive and the data could be treated by software in LITT research. Combining thermoeouple to measure deep tissue temperature, it would have a better effect.