补肾安胎方及其不同组分对胚泡着床障碍小鼠雌、孕激素及其受体的影响

Effect of Bushen Antai Recipe and Its Various Compositions on Endometrial Sexual Hormones and Receptors in Mice with Blastocyst Implantation Dysfunction

目的比较补肾安胎方、补肾方及活血方对胚泡着床障碍小鼠子宫内膜容受性的影响及其机制。方法采用吲哚美辛诱导小鼠胚泡着床障碍模型,从妊娠第1天起分别予补肾安胎方、补肾方、活血方治疗。于妊娠第5、6天处死小鼠,留取血清及子宫标本。采用放射免疫法检测血清雌激素(E2)和孕激素(P)的含量;采用免疫组织化学方法检测子宫雌、孕激素受体(ER、PR)的表达。结果补肾安胎方可以提高妊娠第6天血清P及E2水平,并且促进着床位点ER及PR的表达。而补肾方和活血方虽有轻度促进ER、PR表达作用,但其治疗作用弱于补肾安胎方。结论将补肾法和活血法结合起来的补肾安胎方可以通过对E2、P、ER、PR的影响,从更大程度上改善着床障碍小鼠子宫内膜容受性,促进胚泡着床。

Objective To compare the effects and mechanisms of Bushen Antai Recipe （BAR） and its different compositions, Bushen Recipe （BSR, the Shen-nourishing part） and Huoxue Recipe （HXR, the bloodactivating part） on the endometrial receptivity in mice with blastocyst implantation dysfunction （BID）. Methods Model mice of BID induced by indomethacin were treated respectively with BAR, BSR and HXR from the first day of pregnancy, and killed on day 5 or 6. Samples of their serum and uterine were collected for detecting serum estradiol （E2） , progesterone （P） contents using radioimmunoassay; and endometrial expression levels of their receptors, ER and PR, using immunohistochemistry. Results Serum levels of E2 and P, and endometrial expression of ER and PR increased significantly on day 6 in mice after treated by BAR, but in those treated by BSR and HXR, the improvements were significantly lesser. Conclusion BAR, by combined application of both Shen-nourishing and blood-activating methods, could impact E2, P, ER and PR to improve the endometrial receptivity to a higher extent in BID mice so as to promote embryo implantation.