用微小RNA芯片筛选与系统性硬化病相关的微小RNA

Screening microRNAs related to systemic scleroderma with microRNA array

目的筛选、分析与系统性硬化病发病相关的微小RNA（microRNA）。方法用包含924个microRNA的芯片，检测系统性硬化病患者皮损和正常人皮肤中microRNA的差异，用生物信息学方法检索出差异microRNA调控的靶基因，再筛选出其靶基因中与硬化病相关的基因，分析预测与系统性硬化病相关的microRNA。结果与正常人比较，系统性硬化病患者皮损中发现24个差异表达的microRNA，9条表达上调，15条下调。检索后发现，hsa—miR-206、hsa—let-7g、hsa—miR-133a、hsa—miR-125b、hsa—miR-40—5D、hsa—miR-23b调控的靶基因与硬化病发病相关，特别是hsa—miR-206，其调控的15条靶基因与硬化病的发病密切相关。结论系统性硬化病患者皮损中存在与其发病相关的microRNA，其中hsa—miR-206等6条microRNA可能与硬化病的发病相关。

Objective To screen, analyze and predict microRNAs （miRNAs） related to systemic scleroderma （SSc）. Methods Differentially expressed miRNAs between tissue samples from 3 patients with SSc and 3 normal human controls were screened with a gene chip including 924 miRNAs. Target genes regulated by differentially expressed miRNAs were searched with bioinformatics method. Finally, miRNAs related to SSc were predicted. Results There were 24 miRNAs differentially expressed between tissue samples of SSc and normal controls, including 9 up-regulated miRNAs and 15 down-regulated miRNAs. Literature review disclosed that SSc was associated with target genes regulated by hsa-miR-206, has-let-7g, hsa-miR-133a, hsa-miR-125b, hsa-miR-40-Sp and hsa-miR-23b. In particular, 15 target genes regulated by bsa-miR-206 were closely corre- lated with the pathogenesis of SSc. Conclusions In lesions of SSc, there is an expression of miRNAs related to the pathogenesis of SSc, which may include hsa-miR-206 as well as 5 other miRNAs.