基于叶绿体四个DNA片段联合分析探讨山茶属长柄山茶组、金花茶组和超长柄茶组的系统位置与亲缘关系

Phylogeny of Camellia sects. Longipedicellata,Chrysantha and Longissima(Theaceae) Based on Sequence Data of Four Chloroplast DNA Loci

为探讨山茶属茶亚属(Camellia subgen.Thea)中长柄山茶组(sect. Longipedicellata)、金花茶组(sect. Chrysantha)和超长柄茶组(sect. Longissima)的系统位置和亲缘关系,本研究选取了该属4个亚属11组28个种及2个外类群的材料,对这些材料的叶绿体4个DNA片段(rpl16、psbA-trnH、trnL-F和rpl32-trnL)进行了测序,运用邻接法(neighbor-joining)、最大简约法(maximum-parsimony)和贝叶斯推断(Bayesian inference)对获得的序列进行了联合矩阵分析,并构建基因树。基因树的拓扑结构显示:1)金花茶组包括3个平行的支系,并且长柄山茶组的模式种长柄山茶(Camellia longipedicellata)嵌于其中一个支系,因而金花茶组可能是一个并系或多系类群;2)长柄山茶与越南分布的金花茶组种类在分子系统树上构成一个单系支,暗示了长柄山茶组和金花茶组之间可能具有紧密的亲缘关系;3)超长柄茶组不是一个单系类群,该组的河口超长柄茶(C.hekouensis)位于系统树的基部,与山茶属其余种构成姐妹群。由于缺乏更广泛取样的分析,超长柄茶在山茶属中的系统位置仍然不明确,超长柄茶组与长柄山茶组的亲缘关系问题也没有得到解决。

We focused on the systematic positions and relationships of three sections of Camellia subgen.Thea of Theaceae,i.e.,sect.Longipedicellata,sect.Chrysantha （golden camellias） and sect.Longissima by using four chloroplast DNA regions （rpl16,psbA-trnH,trnL-F rpl32-trnL）. We sampled 28 species representing four subgenus,11 sections in Camellia and two outgroups. Combined analyses of chloroplast DNA sequence data sets are performed with the neighbor-joining,maximum-parsimony and Bayesian inference methods,and the gene trees are constructed. The topologies of gene trees revealed that：1） sect. Chrysantha is paraphyletic or polyphyletic,containing three parallel lineages and Camellia longipedicellata （type of sect.Longipedicellata） nested inside; 2） all four species from Vietnam,together with C. longipedicellata,forming a well-supported monophyletic clade,which implies the close relationship between sect.Longipedicellata and sect.Chrysantha; and 3） sect.Longissima is not monophyletic because C.hekouensis is the sister to the rest of Camellia species. The systematic position of C.longissima and the relationship between sect.Longissima and sect.Longipedicellata are unsolved.