重组质粒pZeoSV2(+)/CpG-HBcAg(ISS)对Balb/c小鼠的免疫作用

Immune effects of recombinant plasmid pZeoSV2(+)/CpG-HBcAg(ISS) action on Balb/c mouse

目的探讨重组质粒pZeoSV2(+)/CpG-HBcAg(ISS)对Balb/c小鼠免疫的作用。方法构建真核表达质粒pZeoSV2(+)/CpG-HBcAg(ISSb,ISSc),将其免疫Balb/c小鼠,ELISA法检测免疫后小鼠血清中HBcAb、IFN-γ、IL-2、IL-12、IL-4和IL-10的含量。结果 pZeoSV2(+)/CpG-HBcAg(ISSb,ISSc)重组质粒均能诱导Balb/c小鼠产生特异性抗体HBcAb,pZeoSV2(+)/CpG-HBcAg(ISSb)组较pZeoSV2(+)/CpG-HBcAg(ISSc)组产生的抗-HBc效价显著增高(P<0.01),且其免疫Balb/c小鼠后能使Th1型细胞因子IFN-γ、IL-2和IL-12的表达增强,抑制Th2型细胞因子IL-4和IL-10的产生。结论 pZeoSV2(+)/CpG-HBcAg(ISSb,ISSc)重组质粒对小鼠HBcAb的产生具有明显的促进作用,含CpG-HBcAg(ISSb)的重组质粒能够明显增强小鼠血清Th1型细胞因子的表达,抑制Th2型细胞因子的表达。

CpG motif can induce strong Thl response, which is necessary for chronic hepatitis B in the study, thus we explore the immune effects of recombinant plasmids pZeoSV2 （＋）/CpG-HBcAg （ISS） on of Balb/c mice. Recombinant plasmid pZeoSV2 （＋）/CpG-HBcAg （ISSb, ISSc） was used to immune Balb/c mice, then the serum HBcAb, IFN-γ IL-2, IL-12, IL-4, and IL-10 levels were detected by ELISA. The pZeoSV2 （＋）/CpG-HBcAg （ISSb, ISSc） recombinant plasmids could induce HBcAb production in Balb/c mice. Furthermore, anti-HBc titers of pZeoSV2（＋）/CpG-HBcAg （ISSb） group were obviously higher than that of pZeoSV2 （＋）/CpG-HBcAg （ISSc） group （P 〈 0.01）. The result showed that high levels of Thl type cytokines including IL-2, IFN-γ and IL-12 were be induced in pZeoSV2 （＋）/CpG- HBcAg （ISSb） recombinant plasmid groups, while the levels of Th2 type cytokines such as IL-4 and IL-10 were inhibited obviously. All the results suggest that pZeoSV2（＋）/CpG-HBcAg （ISSb, c） recombinant plasmid plays a crucial role on HBcAb production in Balb/c mice, and could induce the expression of Thl type cytokines, whereas inhibited the expression of Th2 type cytokines.