摘要
目的通过检测IL-19在结核病患者外周血中的表达,探讨IL-19在肺结核发病机制中的作用。方法研究对象包括初诊肺结核患者34例和正常对照50例,RT-PCR检测外周血单核细胞中IL-19mRNA的表达,酶联免疫吸附法测定外周血清IL-19的水平。结果IL-19在结核病患者外周血单核细胞及血清中的表达均明显高于正常对照组(P<0.01)。结论IL-19可能参与了肺结核的发病。
Objective To investigate the expression of IL-19 in the peripheral blood of tuberculosis patients,and to explore the effects of IL-19 in the pathogenesis of TB.Methods Thirty-four pulmonary tuberculosis patients diagnosed firstly and 50 healthy donors were involved in this study.Reverse transcription polymerase chain reaction was used to detect the expression of IL-19 mRNA in peripheral blood mononuclear cells,and the corresponding expression in blood serum was analyzed by enzyme linked immunosorbent assay.Results The expression of IL-19 in TB group was significantly higher than that in healthy control group （P0.01）.Conclusions IL-19 is involved in the pathogenesis of pulmonary tuberculosis,whose role may be critical.
出处
《实用预防医学》
CAS
2010年第2期265-266,共2页
Practical Preventive Medicine
关键词
IL-19
结核病
聚合酶链反应
酶联免疫吸附法
IL-19
Tuberculosis
Polymerase chain reaction
Enzyme linked immunosorbent assay
