摘要
目的探讨CD44不同剪接体在喉癌中的表达及其临床意义。方法收集39例病理分期为Ⅰ～Ⅳ级喉癌样本及癌旁正常组织。应用反转录-聚合酶链反应分析CD44不同剪接体在喉癌及癌旁正常组织中的表达。结果克隆的CD44剪接变异体有123bp,包含一个完整的阅读框,可变剪接区只有变异型剪接外显子6。喉癌组织中CD44v6基因的表达率显著高于癌旁正常组织(P<0.05)。Ⅲ～Ⅳ期的喉癌组织中CD44v6基因表达率与Ⅰ～Ⅱ期比较,差异有统计学意义(P<0.05);发生淋巴结转移的喉癌组织中CD44v6基因表达率与无淋巴结转移的喉癌组织比较,差异有统计学意义(P<0.05);不同病理分级的喉癌组织,其CD44v6基因表达率间差异有统计学意义(P<0.05);CD44v6基因表达率与肿瘤T分级无关(P>0.05)。结论CD44v6在一定程度上参与了肿瘤的发生、发展,是影响预后的因子之一。CD44v6的过表达与肿瘤的侵袭、转移和临床分期有关。对CD44v6的检测不仅能预测患者的预后,还可为肿瘤的个体化治疗直接提供信息。
Objective To investigate the expression and implication of human CD44 variant in laryngeal carcinoma. Methods Specimens from 39 cases of pathologic stage Ⅰ to Ⅳ laryngeal carcinoma and adjacent carcinoma tissues was performed to detect the expression of cell adhesion protein CD44 variants（ CD44 v） in laryngeal cancer and adjacent carcinoma tissues by using reverse transcription-polymerase chain reaction. Results The CD44v sequence possessed 123bp ：with a completed open reading frame. It was predicted to be only variable splicing exon 6 existed in the flexible region. The expression of CD44v6 in laryngeal carcinoma were much higher than that in adjacent carcinoma tissues（ P 〈0.05 ）. The third and fourth stages laryngeal carcinoma had stronger CD44v6 positive than those of the first and second stages （P 〈 0.05 ）. The expression of CD44 protein in laryngeal carcinoma with metastatic lymph node was higher than that in laryngeal carcinoma without metastatic lymph node（P 〈 0.05 ）. The expression of CD44v6 was positively correlated with the pathological classification（P 〈 0.05 ）but not correlated with T classification of laryngeal carcinoma（ P 〉 0.05 ）. Conclusion CD44v6 takes part in the genesis and development of tumor at some certain and is one of prognostic factors. Overexpression of CD44v6 is closely related to tumor invasion, tumor metastasis, and clinical stage. Detecting CD44v6 not only can predict the prognosis of patients, but also provide direct information for individualized therapy for tumor.
出处
《医学综述》
2010年第8期1257-1260,共4页
Medical Recapitulate
