碳酸酐酶Ⅸ与血管内皮生长因子在直肠癌组织中表达及其临床意义

Assessment of vascular endothelial growth factor and carbonic anhydrase-Ⅸ expression in rectal cancer

目的 探讨直肠癌组织中碳酸酐酶Ⅸ（CA-Ⅸ）和血管内皮生长因子（VEGF）的表达及与临床病理的关系.方法 应用蛋白免疫印迹方法 （Western blot）检测104例直肠癌、30例正常直肠组织、30例直肠腺瘤中CA-Ⅸ和VEGF的表达.结果 直肠癌组织中CA-Ⅸ和VEGF阳性表达率分别为72.1%和65.4%,高于正常直肠组织和腺瘤组织（P〈0.05）.CA-Ⅸ的表达与Dukes＇分期及淋巴结转移相关,Dukes＇分期A至C期CA-Ⅸ阳性率下降（P〈0.05）,淋巴结转移组CA-Ⅸ阳性率低于无淋巴结转移组（P〈0.05）.直肠癌组织和系膜组织VEGF的表达与淋巴结转移关系密切,N2组VEGF阳性表达率分别为81.3%和75.0%,高于NO组的53.7%和25.9%（P〈0.05）.CA-Ⅸ和癌组织和系膜组织的VEGF的表达无明显相关（r=0.092和r=-0.292）.结论 CA-Ⅸ和VEGF表达与直肠癌发生、发展和浸润转移有关,但两者作用机制不同.

Objective To explore the expressions and role of carbonic anhydrases-Ⅸ （CA-Ⅸ） and vascular endothelial growth factor （VEGF） in rectal cancer. Methods The expression levels of CA-Ⅸ and VEGF in 104 pathologically diagnosed rectal cancer tissues, 30 normal tissues, and 30 cases of non-neoplastic rectal mucosa were detected by Western blotting. Results The overall positive expression rate of CA-Ⅸ and VEGF in rectal cancer was 72.1% and 65.4%, repeetively, which was higher than that in non-neoplastic tissue（P〈0.05）. The expression of CA-Ⅸ was related to Dukes＇ stage and status of lymph node metastasis. CA-Ⅸ expression positive rate was decreased with the progression of the Dukes＇ stage from A to C. CA-Ⅸ expression in patients with lymph node negative involvement was higher than that in lymph node involvement, 85.2% vs 58.8%, 56.3%, respectly, with the difference being statistically significant,P〈0.05. The expression of VEGF was related to status of lymph node metastasis. VEGF expression in carcinoma tissues of patients with lymph node involvement （81.3%） was significandy higher than that in lymph node negative involvement （53.7%） ,χ^2=6.88, P〈0.05, and VEGF expression in mesorectal tissues with lymph node involvement （75.0%） was significantly higher than that in lymph node negative involvement （25.9%）,χ^2=14.52, P〈0.05. There was no positive association between CA-Ⅸ and VEGF. Conclusion The hypoxia and angiogenesis are helpful to the judgment of prognosis.