内皮抑素联合放疗对lewis肺癌小鼠中缺氧诱导因子-1α和血管内皮生长因子的影

The effect of rh-endostatin in combination with radiotherapy on the HIF-1α and VEGF expression in mice with lung cancer

目的 观察重组人血管内皮抑制素（YH-16）对不同放疗处理的lewis肺癌小鼠缺氧诱导因子（HIF）-1α和血管内皮生长因子（VEGF）的影响.方法 将60只lewis肺癌细胞小鼠随机分为5组：空白对照组、大剂量单次放疗组、小剂量分次放疗组、大剂量单次放疗联合YH-16组、小剂量分次放疗联合YH-16组.作相应处理后绘制肿瘤生长曲线,计算抑瘤率,用免疫组织化学测定各组HIF-1α和VEGF的阳性表达,并对HIF-1α、VEGF和抑瘤率进行相关分析.结果 抑瘤率和HIF-1α及VEGF表达呈负相关（r分别为-7.823、-8.314,P〈0.05）,而HIF-1α与VEGF表达则无明显线性关系.结论 放疗前使用YH-16,可降低lewis肺癌小鼠组织中HIF-1α和VEGF的表达,使放疗敏感性增加.

Objective To study the effect of rh-endostatin on the hypoxia-inducible factor 1α （HIF-1α） and vascular endothelial growth factor （VEGF） expression in mice with lung cancer treated with radiotherapy. Methods Sixty rats were randomly divided into five groups： control group, the large dose single-γ-ray irradiation group, the small dose fraction irradiation group, the large dose irradiation ＋ YH-16 group and the low dose irradiation ＋ YH-16 group. Inhibition rate and the tumor growth curve were observed. Immunohistochemisty was adopted to detect the expression of HIF-1α and VEGF. The correlation between the expression of HIF-1α, VEGF and inhibilion rate was analyzed. Results The expression of HIF-1α or VEGF and inhibition was correlated negatively （r=-7.823,-8.314, respectively ,P〈0.05）. There was no significant correlation between the expression of HIF-1α and VEGF. Conclusion Adminis-tration of YH-16 before radiotherapy can inhibit the HIF-1α and VEGF expression in mice with lung canc-er, and effectively increase the radiosensitivity of lung cancer,.