EZH2和Bmi-1基因在膀胱移行细胞癌组织中的表达及其意义

The significance and mRNA expression of EZH2 and Bmi-1 genes in transitional cell carcinoma of the bladder tissue

目的观察EZH2和Bmi—1基因在膀胱正常组织和癌组织中的表达及在膀胱肿瘤发生和发展中的作用。方法应用逆转录-聚合酶链反应（RT—PCR）和实时定量PCR的方法，检测EZH2和Bmi。1基因在膀胱正常黏膜（共15例）和膀胱癌中（共70例）的相对表达量，以膀胱癌T24细胞株作为阳性对照。结果在膀胱正常黏膜中，EZH2和Bmi-1基因的mRNA不表达或低表达。而在膀胱癌组织中，两者的表达量明显高于对照组，差异有统计学意义（P〈0．01）。EZH2基因在低度恶性倾向尿路上皮乳头状肿瘤（PUNLMP）组、低分级组和高分级组的表达量分别为：0．1082±0．1880、0．0740±0．0984、0．1885±0．2144，与对照组（0．0158±0．0208）比较，P值分别为〉0．05、〈0．01和〈0．01；在浸润性膀胱癌的表达（0．2069±0．2241）高于表浅性膀胱癌（0．0868±0．1233），其差异有统计学意义（P〈0．01）。Bmi-1基因在PUNLMP组、低分级组和高分级组的表达量分别为：0．6654±0．7668、0．4955±0．4553、0．7986±0．9259与对照组（0．1745±0．0973）比较，P值分别为〉0．05、〉0．05和〈0．01；在浸润性膀胱癌组（0．8368±1．0041）的表达高于表浅性膀胱癌组（0．5505±0．4992），但差异无统计学意义（P〉0．05）。结论EZH2和Bmi-1基因在膀胱癌的发生中起重要作用，并且EZH2与膀胱癌的发生、进展相关。

Objective To investigate the mRNA expression of Enhancer of zeste homolog 2 （EZH2） and B-cell-specific moloney routine leukemia virus insertion site 1 （Bmi-1） in transitional cell carcinoma of the bladder tissue and their roles in the development and progression of bladder carcinoma. Methods The mRNA expression of EZH2 and Bmi-1 in 70 cases of the bladder carcinoma tissue and 15 cases of normal bladder mucosa was detected by RT-PCR and real-time quantitative PCR. The bladder cancer cell line T24 was used as a positive control. Results The expression of EZH2 and Bmi-1 wad undetectable or weak in normal bladder mucosa. The mRNA expression levels of EZH2 and Bmi-1 in the bladder carcinoma were higher than in the normal control group （ P 〈 0.01 ）. The InRNA expression of EZH2 in papillary urothelial neoplasms of low malignant potential （PUNLMP） group, low-grade group and high-grade group was 0. 1082 ±0. 1880, 0. 0740 ±0. 0984 and 0. 1885 ± 0. 2144 respectively, and that in normal control group was 0. 0158±0. 0208（P 〉0.05, 〈0.01 and 〈0.01 respectively）. It was higher in invasive group （0. 2069 ± 0.2241 ） than in superficial group （ 0. 0868 ±0.1233 ） （ P 〈 0.01 ）. The mRNA expression of Bmi-1 in PUNLMP group, low-grade group and high-grade group was 0. 6654 ±0. 7668, 0. 4955±0.4553 and 0.7986±0. 9259, and that in normal control group was （0. 1745±0. 0973 ） （P 〉 0. 05, 〉0.05 and 〈0.01 respectively）. The mRNA expression of Bmi-1 in invasive group （0. 8368 ±1. 0041 ） was higher than in superficial group （0. 5505 ±0. 4992） （P 〉 0.05 ）. Conclusion The expression of both EZH2 and Bmi-1 genes may play an important role in the occurrence of bladder carcinoma, and EZH2 is more closely correlated with the occurrence and progression of bladder carcinoma.