膀胱癌易感性与髓过氧化物酶基因-463G〉A多态性的关系

The association between the -463G 〉 A polymorphism in the MPO gene and bladder cancer risk

目的探讨髓过氧化物酶（MPO）基因-463G〉A多态性与中国苏皖地区汉族人群膀胱癌易感性的关系。方法采用面访填写调查表及采用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法检测年龄和性别匹配的中国苏皖地区汉族354例膀胱癌患者和360例正常人群的MPO-463G〉A基因型。通过分层分析探讨年龄、性别和吸烟对罹患膀胱癌的影响。结果与-463GGI：L较，携带MPO-463GA／AA基因型者可降低罹患膀胱癌的危险性（OR=0．72，95％CI=0．54～0．99）。分层分析结果显示，年龄60岁的男性在吸烟下携带-463GA／AA基因型具有保护作用（年龄60岁：OR=0．37，95％CI=0．22～0．61；男性：0．66，95％CI=0．47—0．97；吸烟：0．64，95％CI=0．41～0．99）。结论MPO-463G〉A多态性可能与我国苏皖地区汉族人群膀胱癌发生有关。

Objective To investigate the association between the - 463G 〉 A polymorphism in the myeloperoxidase （MPO） gene and bladder cancer risk in a southern Chinese population. Methods We obtained information on demographic factors by a personal interview. The polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） using genomic DNA isolated from 354 bladder cancer patients and 360 age and sex matched controls. Stratification analysis by age, sex and smoking status was performed. Results Compared with the -463GG genotype, individuals with GA/AA genotype had a significantly decreased risk of bladder cancer （OR = 0.72, 95% CI = 0.54-0. 99）. In the stratification analysis, it was found that the decreased risk was more pronounced among younger subjects （age ≤60 years） （0.37, 0.22-0.61 ）, men （0.66, 0. 47-0. 97 ） , smokers （0.64, 0. 41-0.99）. Conclusion MPO - 463 G 〉 A polymorphism may contribute to the etiology of bladder cancer in a southern Chinese population.